The trend for the concomitant prescription of antidepressants and antipsychotics is increasing. This calls for a veracious screening and quantifying method for forensic and clinical use. In this study, a liquid chromatography–tandem mass spectrometry (LC–MS-MS) method was developed and validated for the simultaneous determination and quantification of 38 antidepressants, antipsychotics and relevant metabolites in small volumes (200 μL) of human whole blood. Analytes and deuterated internal standards were extracted using liquid–liquid extraction. The separation, determination and quantification of the analytes were performed using an LC–MS-MS system equipped with an ACQUITY UPLC® BEH Phenyl Column under a positive electrospray ionization mode. After validation, the analytical procedure was proved to be highly sensitive, with a limit of detection ranging from 0.0005 to 1 ng/mL and a lower limit of quantification ranging from 0.002 to 2 ng/mL. Bias and within- and between-run precision were within 14.7% for all analytes. Recoveries were reproducible and those of 35 analytes were >50%. Dilution integrity was evaluated to ensure that the therapeutic and toxic blood concentration ranges of target compounds were fully covered. Finally, this method was applied to authentic whole blood samples collected from two forensic cases, which demonstrated its practical usefulness of providing accurate and comprehensive information concerning the previous medication of the deceased.

中文翻译：

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使用LC-MS-MS同时定量测定血液中38种精神药物和相关代谢物

同时使用抗抑郁药和抗精神病药的趋势正在增加。这需要用于法医和临床的准确筛选和量化方法。在这项研究中，开发了液相色谱-串联质谱（LC-MS-MS）方法，并验证了该方法可同时测定和定量少量（200μL）人全血中的38种抗抑郁药，抗精神病药和相关代谢物。使用液液萃取法提取分析物和氘代内标物。使用配备ACQUITYUPLC®BEH苯基柱的LC-MS-MS系统，在正电喷雾电离模式下进行分析物的分离，测定和定量。验证后，分析程序被证明是高度敏感的，检测限为0.0005至1 ng / mL，定量下限为0.002至2 ng / mL。所有分析物的偏倚以及批内和批间精密度均在14.7％之内。回收率可重现，35种分析物的回收率> 50％。评估稀释完整性以确保完全覆盖目标化合物的治疗性血液和毒性血液浓度范围。最后，该方法应用于从两个法医病例中采集的真实全血样本，证明了其提供有关死者先前用药的准确和全面信息的实际实用性。回收率可重现，35种分析物的回收率> 50％。评估稀释完整性以确保完全覆盖目标化合物的治疗性血液和毒性血液浓度范围。最后，该方法应用于从两个法医病例中采集的真实全血样本，证明了其提供有关死者先前用药的准确和全面信息的实际实用性。回收率可重现，35种分析物的回收率> 50％。评估稀释完整性以确保完全覆盖目标化合物的治疗性血液和毒性血液浓度范围。最后，该方法应用于从两个法医病例中采集的真实全血样本，证明了其提供有关死者先前用药的准确和全面信息的实际实用性。