Abstract Nucleic acids containing genetic information are used as biomarkers for the diagnosis of genetic diseases. Herein, silver nanoparticles were utilized as probes to detect single stranded nucleic acids by self-assembly of DNA nanomaterials by spectrophotometry. The target DNA specifically triggered two hairpin DNA molecules to hybridize, generating linear double stranded DNA. This approach effectively prevents silver nanoparticles from aggregating in aqueous solutions of NaCl. Otherwise, silver nanoparticles aggregate without target DNA and hairpin DNA due to the low stability of double stranded DNA. The sensor response was shown to be proportional to the target DNA concentration from 5 to 200 nM with a detection limit equal to 5 nM. This simple platform for the specific determination of nucleic acids without enzymes, labels, and modification and demonstrates great prospects for bioanalysis.

中文翻译：

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用自组装发夹 DNA 和银纳米粒子分光光度法测定单链 DNA

摘要 含有遗传信息的核酸被用作诊断遗传疾病的生物标志物。在此，银纳米粒子被用作探针，通过分光光度法自组装 DNA 纳米材料来检测单链核酸。目标DNA特异性触发两个发夹DNA分子杂交，产生线性双链DNA。这种方法有效地防止了银纳米颗粒在 NaCl 水溶液中聚集。否则，由于双链 DNA 的低稳定性，银纳米粒子会在没有目标 DNA 和发夹 DNA 的情况下聚集。传感器响应显示与目标 DNA 浓度成正比，从 5 到 200 nM，检测限等于 5 nM。这个简单的平台用于特异性测定核酸，无需酶、标签、