ABSTRACT

Flavonoids, among the major bioactive ingredients in many traditional Chinese medicines, have been widely considered due to their pharmacological and biochemical effects: antioxidant, anti-vascular disease and anti-inflammatory effects. To get a better understanding of the role of flavonoids in medicine, food safety and public health, valid and sensitive methods for their pretreatment and determination in different matrices are necessary. This review provides a comprehensive summary of flavonoid pretreatment and analytical methods in different samples since 2013. Pretreatment technologies include simple methods: precipitation protein and acid hydrolysis, and more elaborated ones: solid-liquid extraction, liquid-liquid extraction, solid-phase extraction, matrix solid phase dispersion, and different micro-extraction methods. Determination methods include liquid chromatography, gas chromatography, supercritical fluid chromatography, and electrochemical methods. Moreover, we discuss and compare the strengths and weaknesses of the different pretreatment and analytical methods and suggest a prospect.

摘要

黄酮类化合物是许多中药的主要生物活性成分之一，因其具有抗氧化、抗血管疾病和抗炎等药理和生化作用而被广泛认为。为了更好地了解黄酮类化合物在医学、食品安全和公共卫生中的作用，需要采用有效和灵敏的方法对其在不同基质中进行预处理和测定。本综述对2013年以来不同样品中黄酮类化合物的预处理和分析方法进行了全面总结。预处理技术包括简单的方法：沉淀蛋白和酸水解，以及更精细的方法：固液萃取、液液萃取、固相萃取、基质固相分散，以及不同的微萃取方法。测定方法包括液相色谱法、气相色谱法、超临界流体色谱法和电化学方法。此外，我们讨论和比较了不同预处理和分析方法的优缺点，并提出了展望。