Abstract

Lotus corniculatus L. (Fabaceae) is widely grown in Egypt. It has a great history of folkloric medicinal uses. All fractions of aerial parts of L. corniculatus L. showed significant antioxidant and immunostimulant activities and could strongly induce lymphoproliferation. However, the light petrol fraction had antifungal activity against C. neoformans with IC₅₀ value (<8 µg/mL) and exhibited strongest in-vitro antiprotozoal activity against protozoan parasites belonging to the genera Trypanosoma with IC₅₀ value (0.98 µg/mL) and Plasmodium (with 100% inhibition using a sample concentration of 15866.7 ng/mL). This is the first study of the immunostimulant and antiprotozoal activities of genus Lotus. By this approach, it was possible to isolate eight compounds (-)-7,2'-dihydroxy-4'-methoxyisoflavan (vestitol) (1), kaempferol (2), kaempferol 3-O-α-L-rhamnoside (afzelin) (3), kaempferol 3, 7-O-α-L-dirhamnoside (kaempferitin) (4), kaempferol-3-O-[β-D-xylopyranosyl (1″′→2″)-β-D-galactopyranoside] (5), 3-O-[β-D-glucuronopyranosyl] soyasapogenol B (6), kaempferol-3-O-[β-D-xylopyranosyl (1″′→2″)-β-D-galactopyranoside]-7-O-α-L-rhamnopyranoside (7) and 3-O-[α-L-rhamnopyranosyl (1″′→2″)-β-D-galactopyranosyl-(1″→2′)-β-D-glucuronopyranosyl] soyasapogenol B (soyasaponin І) (8).

摘要

Lotuscorniculatus L.（豆科）在埃及广泛种植。它在民间药用方面有着悠久的历史。L. corniculatus L.地上部分的所有部分都显示出显着的抗氧化和免疫刺激活性，并能强烈诱导淋巴增殖。然而，轻质汽油组分对新型隐球菌具有抗真菌活性，IC ₅₀值 (<8 µg/mL)，并表现出最强的体外抗原生动物活性，对属于锥虫属的原生动物寄生虫具有 IC ₅₀值 (0.98 µg/mL)和疟原虫（使用 15866.7 ng/mL 的样品浓度时 100% 抑制）。这是对莲花属免疫刺激剂和抗原生动物活性的首次研究。通过这种方法，可以分离出八种化合物 (-)-7,2'-二羟基-4'-甲氧基异黄烷 (vestitol) ( 1 )、山奈酚 ( 2 )、山奈酚 3-O-α-L-鼠李糖苷 (afzelin ) ( 3 ), 山奈酚 3, 7-O-α-L-地鼠李素 (kaempferitin) ( 4 ), 山奈酚-3-O-[β-D-吡喃木糖基 (1″′→2″)-β-D-吡喃半乳糖苷] ( 5 ), 3-O-[β-D-吡喃葡萄糖醛酸] 大豆皂醇 B ( 6 ), 山奈酚-3-O-[β-D-吡喃木糖基 (1″′→2″)-β-D-吡喃半乳糖苷]- 7-O-α-L-吡喃鼠李糖苷 ( 7) 和 3-O-[α-L-吡喃鼠李糖基 (1″′→2″)-β-D-吡喃半乳糖基-(1″→2′)-β-D-吡喃葡萄糖基] 大豆皂苷醇 B (soyasaponin І) ( 8 ) .