Abstract

The study was designed to evaluate antigenotoxic effect of methanol Teucrium arduini and Teucrium flavum extracts against mitomycin C (MMC)-induced chromosome and DNA damage in vitro. Cytokinesis-block micronucleus (CBMN) and comet assays were used to investigate effect of plant extracts in different concentrations (125, 250, 500 and 1000 µg/mL) on human peripheral blood lymphocytes (PBLs). The obtained results showed that the all tested concentrations of T. arduini and the highest concentration of T. flavum significantly reduced the MMC-induced micronucleus (MN) frequency in comparison to positive control (only MMC). There were significantly negative correlations between the extracts concentrations and MN frequencies (Pearson, r = −0.905, p = 0.0001 for T. arduini; r = −0.861, p = 0.0001 for T. flavum). The extracts of both plants further lowered the MMC-decreased nuclear division index (NDI) in a dose dependent-manner (Pearson, r = −0.837, p = 0.001 for T. arduini; r = −0.598, p = 0.040 for T. flavum), but significantly only in the highest concentration (1000 µg/mL). Comet assay showed that extracts reduced MMC-increased genetic damage index (GDI), significantly in the concentrations of 500 and 1000 μg/mL, in comparison with positive control. Based on our results, it can be concluded that methanol T. arduini and T. flavum extracts possess protective proapoptotic and antigenotoxic effect which is indication of their medicinal relevance and use in treatment.

摘要

该研究旨在评估甲醇Teucrium arduini和Teucrium flavum提取物对丝裂霉素 C (MMC) 诱导的体外染色体和 DNA 损伤的抗原毒性作用。细胞分裂阻滞微核 (CBMN) 和彗星试验用于研究不同浓度 (125、250、500 和 1000 µg/mL) 的植物提取物对人外周血淋巴细胞 (PBL) 的影响。所得结果表明，T. arduini的所有测试浓度和T. flavum的最高浓度与阳性对照（仅 MMC）相比，显着降低了 MMC 诱导的微核（MN）频率。提取物浓度和 MN 频率之间存在显着负相关（Pearson，r = -0.905， 对于T. arduini ， p = 0.0001 ；对于T. flavum ， r = -0.861，p  = 0.0001 ）。两种植物的提取物以剂量依赖性方式进一步降低 MMC 降低的核分裂指数 (NDI)（Pearson，r = -0.837，对于 T. arduini，p  = 0.001；对于T. arduini，r = -0.598，p  = 0.040 。黄酮)，但仅在最高浓度 (1000 µg/mL) 时显着。彗星试验表明，与阳性对照相比，提取物在 500 和 1000 μg/mL 的浓度下显着降低了 MMC 增加的遗传损伤指数 (GDI)。根据我们的结果，可以得出结论，甲醇T. arduini和T. flavum提取物具有保护性促凋亡和抗原毒性作用，这表明它们的药用相关性和治疗用途。