Wood vinegar wastewater (WVWW) is the main by-product of biomass pyrolysis process, which is more suitable to use anaerobic digestion (AD) to achieve energy recovery due to its large amount of organic matter. In this study, the up-flow anaerobic sludge bed (UASB) reactor was used to investigate the continuous anaerobic transformation of WVWW with gradient concentrations (0.3, 0.675, 1, 2, 3, 4, 5, 6, and 7 g COD/L). Then, the changes of microbial community, diversity index and functional gene were analyzed in detail. The results revealed that WVWW showed good AD performance in continuous fermentation. WVWW of organic loading rate (OLR) of >8.58 g COD/L⋅d showed severe inhibition on biodegradability and methane production, which is mainly due to the toxic substances as compared with the control group. The bacterial communities were dominated by phyla of Chloroflexi, Firmicutes, Proteobacteria, Acidobacteria, Synergistetes, and Actinobacteria. The gene abundances related to energy production, carbohydrate transport and metabolism were relatively high, which are mainly responsible for carbon forms conversion and carbohydrate degradation. This study will provide a basis for the screening and enrichment of functional bacteria and genes.

中文翻译：

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热解木醋废水的连续厌氧消化：微生物多样性和功能基因预测

木醋液废水（WVWW）是生物质热解过程的主要副产物，其有机物含量大，更适合采用厌氧消化（AD）实现能量回收。在本研究中，上流式厌氧污泥床 (UASB) 反应器用于研究 WVWW 在梯度浓度（0.3、0.675、1、2、3、4、5、6 和 7 g COD/ L)。然后详细分析了微生物群落、多样性指数和功能基因的变化。结果表明，WVWW在连续发酵中表现出良好的AD性能。WVWW 的有机负荷率 (OLR) > 8.58 g COD/L⋅d 对生物降解性和甲烷产生有严重抑制，这主要是由于与对照组相比有毒物质。细菌群落主要由 Chloroflexi、Firmicutes、Proteobacteria、Acidobacteria、Synergistetes 和 Actinobacteria 的门组成。与能量产生、碳水化合物转运和代谢相关的基因丰度相对较高，主要负责碳形式转化和碳水化合物降解。该研究将为功能细菌和基因的筛选和富集提供依据。