Magnolia lucida (Magnoliaceae) is classified as an endangered species by the International Union for Conservation of Nature. It has high commercial value owing to its attractive tree shape and flowers. We adopted an excellent genotype of M. lucida as the parent material and established a mini-cut orchard through grafting to provide trunk shoots explants over the long-term. Optimal sterilization was achieved using a combination of 75% ethanol for 30 s, one percent benzalkonium bromide for five minutes, and 0.1% mercuric chloride for five minutes. Modified Murashige and Skoog medium (ML) was the optimal medium for the growth of M. lucida. Addition of one mg/L of 6-benzyl adenine (BA) and 0.05 mg/L of α-naphthaleneacetic acid (NAA) to the medium increased the shoot induction rate to 95.56%, and the ML medium containing 0.4 mg/L BA and 0.04 mg/L NAA achieved the maximum multiplication rate (284.56%). Dark treatment for seven days, followed by continuous light treatment could better resolve the challenge of difficult rooting in M. lucida plants. Using random amplified polymorphic DNA and inter simple sequence repeat markers, we confirmed the genetic uniformity and stability of the regenerated plants. Our protocol should be helpful for the propagation and conservation of this endangered plant.

中文翻译：

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玉兰（木兰科）的有效组织培养方案和再生植物的遗传稳定性的确认。

国际自然保护联盟将木兰（Magnoliaceae）列为濒危物种。由于其迷人的树形和花朵，它具有很高的商业价值。我们采用优良的M. lucida基因型作为母本，并通过嫁接建立了一个小型切果园，以长期提供树干新芽。使用75％的乙醇30秒钟，1％的苯扎溴铵5分钟和0.1％的氯化汞5分钟的组合可以实现最佳的灭菌效果。改良的Murashige and Skoog培养基（ML）是灵芝分支杆菌生长的最佳培养基。向培养基中添加1 mg / L的6-苄基腺嘌呤（BA）和0.05 mg / L的α-萘乙酸（NAA），可将枝条诱导率提高到95.56％，而含有0.4 mg / L BA和0.04 mg / L NAA达到最大繁殖率（284.56％）。暗处理7天，然后进行连续的光处理，可以更好地解决在lucida lucida植物中难以生根的挑战。使用随机扩增的多态性DNA和简单的序列间重复标记，我们证实了再生植物的遗传一致性和稳定性。我们的协议应有助于这种濒危植物的繁殖和保存。