Few studies focused on the effects of iron on characterizing alterations of metabolic processes in neonatal piglets. In the present study, 16 neonatal piglets were randomly assigned to two groups. In the first group piglets were given an intramuscularly injection of iron dextran at 150 mg as a positive control (CON) and the second group were not supplemented with iron as a negative control for iron deficiency (ID). At day 8, iron status, serum biochemical parameters, serum metabolome, hepatic histology, and hepatic expression of genes for the metabolism were analyzed. Results indicated that piglets without iron supplementation had significantly reduced iron values and increased blood urea nitrogen concentrations at day 8 (p < 0.05). Analysis of serum metabolome revealed that concentrations of serum lysine, leucine, tyrosine, methionine, and cholesterol were significantly decreased while concentrations of 3-Methyldioxyindole, chenodeoxycholate acid, indoleacetic acid, icosadienoic acid, phenylpyruvic acid, pantothenic acid, ursocholic acid, and cholic acid were significantly increased in iron deficient piglets (p < 0.05). Furthermore, expressions of cyp7a1 and the urea cycle enzyme (ornithinetranscarbamoylase and argininosuccinate synthetase) were significantly increased in iron deficient pigs (p < 0.05). The present experimental results indicated that neonatal piglets without iron supplementation drop to borderline anemia within 8 days after birth. Iron deficiency led to a series of metabolic changes involved in tyrosine metabolism, phenylalanine metabolism, bile secretion, primary bile acid biosynthesis, steroid biosynthesis, and upregulated activities of the urea cycle enzymes in the liver of neonatal piglets, suggesting early effects on metabolic health of neonatal piglets.

中文翻译：

---

缺铁对新生仔猪血清代谢组，肝组织学和功能的影响。

很少有研究关注铁对新生仔猪代谢过程变化的影响。在本研究中，将16只新生仔猪随机分为两组。在第一组中，仔猪肌肉注射右旋糖酐铁150 mg作为阳性对照（CON），第二组不补充铁作为缺铁（ID）的阴性对照。在第8天，分析铁的状态，血清生化参数，血清代谢组，肝组织学和用于代谢的基因的肝表达。结果表明，不添加铁的仔猪在第8天时铁含量显着降低，血尿素氮浓度升高（p<0.05）。血清代谢组学分析表明，血清赖氨酸，亮氨酸，酪氨酸，蛋氨酸和胆固醇的浓度显着降低，而3-甲基二氧吲哚，鹅去氧胆酸，吲哚乙酸，二十二碳二烯酸，苯丙酮酸，泛酸，熊胆酸和胆酸的浓度明显降低。缺铁仔猪的体重显着增加（p <0.05）。此外，缺铁猪的cyp7a1和尿素循环酶（鸟氨酸转氨甲酰酶和精氨琥珀酸合成酶）的表达显着增加（p<0.05）。目前的实验结果表明，不补充铁的新生仔猪在出生后8天内下降为边缘性贫血。缺铁导致酪氨酸代谢，苯丙氨酸代谢，胆汁分泌，初级胆汁酸生物合成，类固醇生物合成以及新生仔猪肝脏中尿素循环酶活性上调的一系列代谢变化，提示对仔猪代谢健康的早期影响新生仔猪。