Guinea pigs (Cavia porcellus) have been used in research since the 19th century to collect data due to their physiological similarities to humans. Today, animals perform a vital role in experiments and concerns for laboratory animal welfare are enshrined in the 3R framework of reduction, refinement and replacement. This case study explores a refinement in humane euthanasia of guinea pigs via the use of an irreversible penetrating spring-loaded captive bolt (CB). Penetrating spring-loaded CB stunning for euthanasia (CBE) was performed on 12 guinea pigs with the parameters for humane slaughter of production animals in order to assess the suitability of this method of euthanasia in contrast to blunt force trauma (BFT). All 12 of the guinea pigs were rendered immediately unconscious with excellent experimental tissue quality collection, high repeatability of results and operator (n = 8) preference over BFT. Overall, CBE in guinea pigs appears to be a feasible refinement for animal welfare, human preference and improved tissue quality for experimental collection in settings where uncontaminated tissues are required.

中文翻译：

---

豚鼠（Cavia porcellus）的人道安乐死，具有穿透性的弹簧式固定螺栓。

豚鼠（Cavia porcellus由于其与人类的生理相似性，自19世纪以来一直用于研究中以收集数据。如今，动物在实验中起着至关重要的作用，而对实验室动物福利的关注已被纳入减少，完善和替代的3R框架中。本案例研究通过使用不可逆穿透弹簧加载系留螺栓（CB）探索了对豚鼠人道安乐死的改进。为了评估这种安乐死方法与钝力外伤（BFT）相比的适用性，对12头豚鼠进行了穿透性弹簧加载的CB安乐死击晕试验（CBE），该参数具有人道屠宰生产动物的参数。通过出色的实验组织质量采集，所有12只豚鼠立即失去知觉，n = 8）优先于BFT。总体而言，在需要未污染组织的环境中，豚鼠中的CBE似乎是动物福利，人类偏爱和改善组织质量的可行改进，以用于实验收集。