In this study, the effect of different feeding frequency (TA: twice/day, TB: 3 times/day, TC: 4 times/day) on the feeding, growth, reproductive performance, body composition and digestive enzyme activity of hepatopancreas of Penaeus monodon broodstock was compared. The living Nereis succinea were used to feed the P. monodon broodstock for 8 weeks to explore the most appropriate feeding frequency during the breeding period. The results showed that the daily feeding amount of P. monodon broodstock increased with the increase of feeding frequency, and the daily feeding amount of TA group was significantly lower than that of TB and TC group (p < .05), but the difference between TB group and TC group was not significant (p > .05). There was no significant difference on the spawning amount, the number of the nauplius I individuals, the hatchability of nauplius, the number of the zoaea larva I individuals, the metamorphosis rate, the gonadal index and the first spawning time when Penaeus monodon broodstock was fed in different frequency (p > .05). The relative fecundity of TA group was significantly lower than that of TB and TC group (p < .05), and the difference between TB and TC group was not significant (p > .05). The spawning interval of TA group was significantly higher than that of TB and TC group (p < .05), and the difference between TB and TC group was not significant (p > .05). The contents of moisture, crude protein, crude fat and ash of the P. monodon broodstock body were not affected by the increase of the feeding frequency (p > .05). The activities of trypsin and lipase in TA group were significantly lower than those in TB and TC group (p < .05), while the activities of pepsin and amylase in different experimental groups were not significantly different (p > .05). According to the results of this study and the actual situation of production, it is suggested that the suitable feeding frequency of nutrition enhancement period during the reproduction of P. monodon broodstock is 3 times/day in the condition of satiation.

中文翻译：

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饲喂频率对斑节对虾亲虾生长，繁殖性能，体成分和消化酶活性的影响

在这项研究中，不同饲喂频率（TA：两次/天，TB：3次/天，TC：4次/天）对对虾肝胰腺的摄食，生长，生殖性能，身体成分和消化酶活性的影响比较了斑节对虾亲虾。用活的Nereis succinea饲喂斑节对虾亲虾8周，以探索繁殖期间最合适的饲喂频率。结果表明，斑节对虾的日饲喂量随着饲喂次数的增加而增加，TA组的日饲喂量明显低于TB和TC组（p <.05），但TB组和TC组之间的差异不显着（p  > .05）。斑节对虾亲虾的产卵量，无节幼体I个体的数量，无节幼体的孵化率，zoaea幼体I个体的数量，变态率，性腺指数和第一次产卵时间均无显着差异。以不同的频率（p  > .05）。TA组的相对生殖力显着低于TB和TC组（p  <.05），而TB和TC组之间的差异无统计学意义（p  > .05）。TA组的产卵间隔明显高于TB和TC组（p <.05），而TB和TC组之间的差异不显着（p  > .05）。斑节对虾亲鱼体的水分，粗蛋白，粗脂肪和灰分的含量不受饲喂频率增加的影响（p  > .05）。TA组的胰蛋白酶和脂肪酶活性明显低于TB和TC组（p  <.05），而不同实验组的胃蛋白酶和淀粉酶的活性无明显差异（p  > .05）。根据研究结果和生产的实际情况，建议斑节对虾繁殖过程中营养增强期的适宜饲喂频率 在饱足条件下，亲鱼每天3次。