Hydroxyapatite-Incorporated Composite Gels Improve Mechanical Properties and Bioactivity of Bone Scaffolds.,Macromolecular Bioscience

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Hydroxyapatite-Incorporated Composite Gels Improve Mechanical Properties and Bioactivity of Bone Scaffolds.
Macromolecular Bioscience ( IF 4.4 ) Pub Date : 2020-08-05 , DOI: 10.1002/mabi.202000176
Sanika Suvarnapathaki _{1,

2} , Xinchen Wu _{1,

2} , Darlin Lantigua _{1,

2} , Michelle A Nguyen ₁ , Gulden Camci-Unal _{1,

3}

Affiliation

Reinforcing polymeric scaffolds with micro/nanoparticles improve their mechanical properties and render them bioactive. In this study, hydroxyapatite (HA) is incorporated into 5% (w/v) gelatin methacrylate (GelMA) hydrogels at 1, 5, and 20 mg mL⁻¹ concentrations. The material properties of these composite gels are characterized through swelling, degradation, and compression tests. Using 3D cell encapsulation, the cytocompatibility and osteogenic differentiation of preosteoblasts are evaluated to assess the biological properties of the composite scaffolds. The in vitro assays demonstrate increasing cell proliferation and metabolic activity over the course of 14 d in culture. Furthermore, the scaffolds support osteogenic differentiation of the microencapsulated preosteoblasts. For the in vivo study, the composite scaffolds are subcutaneously implanted in rats for 14 d. The histological staining of the explanted in vivo samples exhibits the functional advantages of the scaffold's biocompatibility, biodegradability, and integration into the existing host tissue. This work demonstrates the enhanced mechanical and biological performance of HA‐gelatin composite hydrogels for bone tissue engineering applications.

中文翻译：

羟基磷灰石复合凝胶可改善骨支架的机械性能和生物活性。

用微/纳米颗粒增强聚合物支架可改善其机械性能并使其具有生物活性。在本研究中，羟基磷灰石 (HA) 以 1、5 和 20 mg mL ^{-1 的}浓度加入 5% (w/v) 的甲基丙烯酸明胶 (GelMA) 水凝胶中浓度。这些复合凝胶的材料特性通过溶胀、降解和压缩测试来表征。使用 3D 细胞封装，评估前成骨细胞的细胞相容性和成骨分化，以评估复合支架的生物学特性。体外测定表明，在培养 14 天的过程中，细胞增殖和代谢活性增加。此外，支架支持微囊化前成骨细胞的成骨分化。在体内研究中，将复合支架皮下植入大鼠体内 14 天。外植体内样品的组织学染色显示了支架的生物相容性、生物降解性和与现有宿主组织整合的功能优势。

更新日期：2020-10-12

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