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Comparative assessment of functional properties, free and bound phenolic profile, antioxidant activity, and in vitro bioaccessibility of rye bran and its insoluble dietary fiber.
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2020-08-04 , DOI: 10.1111/jfbc.13388
Maryam Iftikhar 1 , Huijuan Zhang 1 , Asra Iftikhar 2 , Ali Raza 1 , Majid Khan 1 , Miao Sui 1 , Jing Wang 1
Affiliation  

In cereals, 95% of dietary fiber is associated with phenolic compounds. The present study examined the functional properties, phenolic composition, antioxidant activity, and in vitro bioaccessibility of phenolics and flavonoids present in rye bran (RB) and its insoluble dietary fiber (IDF). Compared to RB, higher functional properties (WHC, WRC, and OHC) were represented by IDF due to its porous structure. The IDF contained lower free but higher bound phenolics and flavonoids content as compared to RB, whereas highest total phenolics (556.6 mg GAE/100 g) and flavonoids (378.3 mg RE/100 g) content were observed in IDF. Results had identified significant differences (p < .05) in phenolic acids composition between RB and IDF determined by HPLC‐MS and the total phenolic acids were higher in IDF. The antioxidant capacity of IDF was higher than RB in DPPH, FRAP, ABTS, and reducing power assay. However, the in vitro phenolics and flavonoids bioaccessibility of IDF was much lower because of its high content of bound phenolics and flavonoids.

中文翻译:


黑麦麸及其不溶性膳食纤维的功能特性、游离和结合酚谱、抗氧化活性和体外生物可及性的比较评估。



在谷物中,95%的膳食纤维与酚类化合物有关。本研究检查了黑麦麸 (RB) 及其不溶性膳食纤维 (IDF) 中酚类和黄酮类化合物的功能特性、酚类成分、抗氧化活性和体外生物可利用性。与RB相比,IDF由于其多孔结构而表现出更高的功能特性(WHC、WRC和OHC)。与 RB 相比,IDF 含有较低但结合的酚类和类黄酮含量,而 IDF 中观察到总酚类(556.6 mg GAE/100 g)和类黄酮(378.3 mg RE/100 g)含量最高。结果发现,通过 HPLC-MS 测定,RB 和 IDF 之间的酚酸组成存在显着差异 ( p < .05),并且 IDF 中的总酚酸较高。在DPPH、FRAP、ABTS、还原力测定中IDF的抗氧化能力均高于RB。然而,由于IDF的结合酚类和类黄酮含量较高,其体外酚类和类黄酮生物可利用性要低得多。
更新日期:2020-10-11
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