Identification of salivary proteins in the whitefly Bemisia tabaci by transcriptomic and LC–MS/MS analyses,Insect Science

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Identification of salivary proteins in the whitefly Bemisia tabaci by transcriptomic and LC–MS/MS analyses
Insect Science ( IF 2.9 ) Pub Date : 2020-08-05 , DOI: 10.1111/1744-7917.12856
Hai-Jian Huang ₁ , Zhuang-Xin Ye ₁ , Gang Lu ₁ , Chuan-Xi Zhang ₁ , Jian-Ping Chen ₁ , Jun-Min Li ₁

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The whitefly Bemisia tabaci is a notorious agricultural pest of many crops worldwide. Although it is thought that B. tabaci secretes saliva into the host plant to counter plant defenses, knowledge on the whitefly salivary proteome is limited. Here, we characterized the gene/protein repertoires of B. tabaci salivary glands and secreted saliva by transcriptomic and liquid chromatography tandem mass spectroscopy analyses. A total of 698 salivary gland-enriched unigenes and 171 salivary proteins were identified. Comparative analysis between the B. tabaci salivary proteins and those of different arthropod species revealed numerous similarities in proteins associated with binding, hydrolysis, and oxidation-reduction, which demonstrates a degree of conservation across herbivorous saliva. There were 74 proteins only identified in B. tabaci saliva, of which 34 were B. tabaci-specific. In addition, 13 salivary proteins, of which 11 were B. tabaci-specific, were differentially regulated when B. tabaci fed on different hosts. Our results provide a good resource for future functional studies of whitefly salivary effectors, and might be useful in pest management.

中文翻译：

通过转录组学和 LC-MS/MS 分析鉴定粉虱 Bemisia tabaci 中的唾液蛋白

粉虱Bemisia tabaci是世界范围内许多作物的臭名昭著的农业害虫。尽管人们认为烟粉虱将唾液分泌到寄主植物中以对抗植物防御，但对粉虱唾液蛋白质组的了解是有限的。在这里，我们通过转录组学和液相色谱串联质谱分析表征了烟粉虱唾液腺和分泌唾液的基因/蛋白质库。共鉴定出 698 个富含唾液腺的 unigenes 和 171 个唾液蛋白。烟粉虱的比较分析唾液蛋白和不同节肢动物物种的唾液蛋白揭示了与结合、水解和氧化还原相关的蛋白质的许多相似之处，这表明食草动物的唾液具有一定程度的保守性。仅在烟粉虱唾液中鉴定出 74 种蛋白质，其中 34 种是烟粉虱特异性的。此外，当粉虱以不同宿主为食时，13 种唾液蛋白（其中 11 种是粉虱特异性的）受到差异调节。我们的结果为粉虱唾液效应物的未来功能研究提供了很好的资源，并且可能对害虫管理有用。

更新日期：2020-08-05

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