Newly developed vaccine strains to prevent foot-and-mouth disease caused by the emerging serotype Asia1 virus were evaluated. To protect against the group (G)-VIII strain, which occurred recently, we produced an infectious cDNA clone of Asia1 Shamir cDNA (Asia1 Shamir-R). In addition, by adding a site 1 epitope of VP1 of the G-VIII lineage virus to this virus, we produced a new virus (Sham GVIII- EPI), and another virus(Sham GVIII-VP1) was replaced with that of G-VIII lineage in the VP1 region of Shamir. Test vaccines were produced using these three types of vaccine virus, and their immunogenicity and protection capabilities were evaluated in mice. Immunized mice were challenged with the Asia1 Shamir or G-VIII virus, and the results show that all the vaccines have similar protective effects. As they showed similar antigenicity, we chose the Shamir-R vaccine. Pigs maintained relatively high neutralizing antibody levels against homologous viruses of the Shamir and G-VII or G-VIII lineage three to four weeks after immunization. However, they formed relatively low levels of antibodies to G-IV and G-V viruses. In conclusion, we produced a vaccine candidate capable of protection against the G-VIII virus in the vaccine experiment for the type Asia1 serotype vaccine. This Shamir-R vaccine virus was found to protect against the viruses of the Asia1 genotype G-VII and G-VIII lineages, which occurred recently in Asia.

对新开发的疫苗菌株进行了预防，以预防由新兴的血清型Asia1病毒引起的口蹄疫。为了防止最近出现的（G）-VIII毒株，我们生产了Asia1 Shamir cDNA（Asia1 Shamir-R）的传染性cDNA克隆。此外，通过在该病毒中添加G-VIII谱系病毒VP1的第1位表位，我们生产了一种新病毒（Sham GVIII-EPI），另一种病毒（Sham GVIII-VP1）被G- Shamir VP1地区的VIII世系。使用这三种类型的疫苗病毒生产了测试疫苗，并在小鼠中评估了它们的免疫原性和保护能力。免疫小鼠受到Asia1 Shamir或G-VIII病毒的攻击，结果表明所有疫苗都具有相似的保护作用。由于它们显示出相似的抗原性，我们选择了Shamir-R疫苗。免疫后三至四周，猪对沙米尔和G-VII或G-VIII世系的同源病毒保持较高的中和抗体水平。但是，它们形成的G-IV和GV病毒抗体水平相对较低。总之，在针对Asia1型血清型疫苗的疫苗实验中，我们生产了能够抵抗G-VIII病毒的候选疫苗。人们发现这种Shamir-R疫苗病毒可以抵抗亚洲最近出现在亚洲的G1 VII和G-VIII基因型的病毒。总之，在针对Asia1型血清型疫苗的疫苗实验中，我们生产了能够抵抗G-VIII病毒的候选疫苗。人们发现，这种Shamir-R疫苗病毒可以防御亚洲最近出现在亚洲的G1 VII和G-VIII基因型的病毒。总之，在针对Asia1型血清型疫苗的疫苗实验中，我们生产了能够抵抗G-VIII病毒的候选疫苗。人们发现这种Shamir-R疫苗病毒可以抵抗亚洲最近出现在亚洲的G1 VII和G-VIII基因型的病毒。