Membrane filters were coated with 10,12-pentacosadiynoic acid (PCDA) then polymerized on the filter for rapid bacterial detection and quantification. The polymerized PCDA (pPDCA)-coated filter changed color in response to Salmonella Typhimurium and Escherichia coli but not to Listeria innocua. The time required for color change of pPCDA-coated filters was determined by a visual panel. A simple linear regression model was generated to fit the observed data and was validated with goodness of fit analysis and residual analysis. The pPCDA-filter method estimated Salmonella Typhimurium populations of 8 to 3 log CFU ml⁻¹ within 1.5–7.5 h, respectively.

膜滤膜上涂有10,12-戊二十二碳二烯酸（PCDA），然后在滤膜上聚合以快速检测和定量细菌。聚合的PCDA（pPDCA）涂层过滤器响应鼠伤寒沙门氏菌和大肠杆菌而改变颜色，但无毒李斯特菌则没有。pPCDA涂层滤光片的颜色变化所需的时间由可视面板确定。生成了一个简单的线性回归模型以拟合观察到的数据，并通过拟合分析和残差分析的优势进行了验证。pPCDA过滤器方法估计在1.5–7.5 h内鼠伤寒沙门氏菌种群分别为8至3 log CFU ml ^-1。