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Detecting and correlating bacterial populations to visual color change of polydiacetylene-coated filters
Talanta ( IF 5.6 ) Pub Date : 2020-08-05 , DOI: 10.1016/j.talanta.2020.121482
Yueyuan Zhang , Paul L. Dawson , Timothy W. Hanks , Julie K. Northcutt , Tzuen-Rong Tzeng , William T. Pennington

Membrane filters were coated with 10,12-pentacosadiynoic acid (PCDA) then polymerized on the filter for rapid bacterial detection and quantification. The polymerized PCDA (pPDCA)-coated filter changed color in response to Salmonella Typhimurium and Escherichia coli but not to Listeria innocua. The time required for color change of pPCDA-coated filters was determined by a visual panel. A simple linear regression model was generated to fit the observed data and was validated with goodness of fit analysis and residual analysis. The pPCDA-filter method estimated Salmonella Typhimurium populations of 8 to 3 log CFU ml−1 within 1.5–7.5 h, respectively.



中文翻译:

检测细菌种群并将其与聚二乙炔涂层过滤器的视觉颜色变化相关联

膜滤膜上涂有10,12-戊二十二碳二烯酸(PCDA),然后在滤膜上聚合以快速检测和定量细菌。聚合的PCDA(pPDCA)涂层过滤器响应鼠伤寒沙门氏菌和大肠杆菌而改变颜色,但无毒李斯特菌则没有。pPCDA涂层滤光片的颜色变化所需的时间由可视面板确定。生成了一个简单的线性回归模型以拟合观察到的数据,并通过拟合分析和残差分析的优势进行了验证。pPCDA过滤器方法估计在1.5–7.5 h内鼠伤寒沙门氏菌种群分别为8至3 log CFU ml -1

更新日期:2020-08-10
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