A novel anaerobic moderately thermophilic bacterium, designated strain 38H-str^T, was isolated from a 12 m deep hot spring of the Kunashir Island shore. Gram-negative cells were non-spore-forming, motile, straight or curved filamentous rods, occasionally forming loops and knots. The strain grew at 20–65 °C and pH range of 4.0–9.0 with an optimum at 50 °C and pH 6.5–7.0. Strain 38H-str^T required 0.5–2.5% NaCl (1.5% is an optimum) for growth. It was a chemoorganoheterotroph, growing on carbohydrates (starch, pullulan, alginate, laminarin, beta-glucan) or peptide mixtures and proteins (peptone, tryptone, gelatin, and α- or β- keratins). Major products of glucose fermentation were acetate, hydrogen, and carbon dioxide. Major cellular fatty acids were iso- and anteiso-C_15:0. Phosphatidylethanolamine, an unidentified phospholipid, and three unidentified polar lipids were detected in cellular lipids fractions. The quinone was MK-7. The size of complete genome of strain 38H-str^T was 3.2 Mb; DNA G+C content was 38.3 mol%. According to 16S rRNA gene sequence and conserved protein sequences phylogenies, strain 38H-str^T represented a deeply branched lineage near the root of the class Bacteroidia. Based on phylogenetic analysis and phenotypic features the novel isolate was assigned to a novel family within the order Bacteroidales for which the name Tenuifilaceae fam. nov. is proposed. Strain 38H-str^T (=DSM 100343^T =VKM B-2964^T) represents the first genus and species Tenuifilum thalassicum gen. nov., sp. nov.

一种新颖的厌氧中度嗜热菌，指定应变38H-STR ^Ť，从国后岛岸边的一个12米深温泉中分离。革兰氏阴性细胞是非孢子形成的，能动的，直的或弯曲的丝状杆，偶而形成环和结。菌株在20–65°C和pH范围为4.0–9.0的条件下生长，最佳温度在50°C和pH值为6.5–7.0的条件下。38H-str ^T菌株需要0.5–2.5％NaCl（1.5％是最佳）才能生长。它是一种化学有机异养生物，在碳水化合物（淀粉，支链淀粉，藻酸盐，层粘连蛋白，β-葡聚糖）或肽混合物和蛋白质（蛋白pe，胰蛋白,、明胶和α-或β-角蛋白）上生长。葡萄糖发酵的主要产物是乙酸盐，氢气和二氧化碳。细胞内主要脂肪酸为异-和反-C _15：0。在细胞脂质组分中检测到磷脂酰乙醇胺，一种未鉴定的磷脂和三种未鉴定的极性脂质。醌是MK-7。菌株38H-str ^T的完整基因组大小为3.2 Mb；DNA G + C含量为38.3mol％。根据16S rRNA基因序列和保守的蛋白质序列系统发育，菌株38H-str ^T代表了类细菌根部附近的深分支谱系。基于系统发育分析和表型特征，该新分离株被分配到细菌科的一个新科中，其名称为Tenuifilaceae fam。十一月 被提议。菌株38H-str ^T（= DSM 100343 ^T = VKM B-2964^T）代表第一个属Tenalifilum thalassicum属。十一月，sp。十一月