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Magnetic field as a trigger of carotenoid production by Phaffia rhodozyma
Process Biochemistry ( IF 3.7 ) Pub Date : 2020-11-01 , DOI: 10.1016/j.procbio.2020.08.001
Pedro Garcia Pereira da Silva , Daniel Prescendo Júnior , Luisa Sala , Janaina Fernandes de Medeiros Burkert , Lucielen Oliveira Santos

Abstract This study aimed to evaluate the influence of magnetic fields (MF) on inoculum cultivation and carotenoid production by Phaffia rhodozyma. The application of MF in the inoculum culture was evaluated (0 m T – control and 30 m T). Cellular concentration increased by 12.8 % after 24 h-culture with MF application compared to the control assay, and this was the best alternative for the preparation of inoculum. Different intervals of MF application were evaluated over 168 h. The highest volumetric carotenoids concentration was achieved by applying MF throughout cultivation, with values of 1146.39 ± 26.18 μg L−1 and carotenoid productivity of 11.94 ± 1.11 μg L−1 h−1 in 96 h. As a result, carotenoid production increased by 59.4 % and carotenoid productivity by 99.3 %. This study is one of the first to consider MF application in carotenoid production using P. rhodozyma as a viable and low-cost alternative for carotenoid production in a shorter cultivation time.

中文翻译:

磁场作为红发夫酵母产生类胡萝卜素的触发器

摘要 本研究旨在评估磁场(MF)对红发夫酵母菌接种物培养和类胡萝卜素产量的影响。评估了 MF 在接种物培养中的应用(0 m T - 对照和 30 m T)。与对照试验相比,使用 MF 培养 24 小时后,细胞浓度增加了 12.8%,这是制备接种物的最佳选择。在 168 小时内评估了 MF 应用的不同间隔。最高体积类胡萝卜素浓度是通过在整个培养过程中应用 MF 实现的,值为 1146.39 ± 26.18 μg L-1,96 小时内的类胡萝卜素生产率为 11.94 ± 1.11 μg L-1 h-1。结果,类胡萝卜素产量增加了 59.4%,类胡萝卜素产量增加了 99.3%。
更新日期:2020-11-01
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