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Analysis of molecular characterizations of beets, Beta vulgaris in response to cyst nematodes, Heterodera schachtii
Physiological and Molecular Plant Pathology ( IF 2.8 ) Pub Date : 2020-12-01 , DOI: 10.1016/j.pmpp.2020.101518
Reza Naderi , Mehdi Nasr Esfahani , Gholamreza Bakhshi Khaniki

Abstract Sugar beet cyst nematode Heterodera schachtii is a serious biotic limiting factor in sugar beet industry. In this study, 70 sugar beet accessions were screened against H. schachtii, of which 30 resistant and susceptible ones were subjected to polymorphic simple sequence repeat (SSR) marker analysis by seven Universal DNA Amplification (UDA) primer pairs randomly. The results showed a very high significant variation between resistant and susceptible sugar beet accessions in response to H. schachtii with 42 fold increase in nematode reproduction rate in susceptible ones. SSR primers produced 155 bands, out of which 91 ones showed polymorphism, indicating 59% genetic diversity. The highest polymorphic bands was of UDA013 primer with 17 and the lowest one in UDA010 with 10 bands. Average number of polymorphic bands per primer was 13 and per genotype 3.03 bands. The UDA015 had the highest polymorphic index content (PIC) of 80%, and the lowest 44% in UDA001 with the mean of 67%. Highest marker index (MI) was in UDA015 with 10.80, and lowest one 6.48 in UDA001, with the mean of 8.68. Cluster analysis using Numerical Taxonomy and Multivariate Analysis System (NTSYS) software, with UPGMA method based on Jacard's similarity matrix, divided the genotypes into 4 major clusters. The dendrogram was consistent with the clustering result of Principal Component Analysis (PCoA). The highest genetic similarity of 91% was between the genotypes 45 and 51, of which both were offspring of a half-brother and half-sister crossing. The results suggested novel sources of resistant to H. schachtii and that SSR marker is a reliable tool for revealing a high levels of polymorphism among the sugar beet genotypes.

中文翻译:

甜菜、甜菜对胞囊线虫、Heterodera schachtii 响应的分子特征分析

摘要 甜菜胞囊线虫 Heterodera schachtii 是甜菜工业中严重的生物限制因子。在本研究中,对 70 个甜菜种质进行了 H. schachtii 筛选,其中 30 个抗性和易感性种质通过 7 个通用 DNA 扩增 (UDA) 引物对随机进行多态性简单序列重复 (SSR) 标记分析。结果表明,响应 H. schachtii 的抗性和易感甜菜种质之间存在非常高的显着差异,易感性甜菜的线虫繁殖率增加了 42 倍。SSR引物产生155条带,其中91条显示多态性,表明59%的遗传多样性。多态性最高的是UDA013引物,有17条,最低的是UDA010,有10条。每个引物的多态性条带平均数为 13,每个基因型 3.03 个条带。UDA015的多态指数含量(PIC)最高,为80%,最低的为UDA001,为44%,平均值为67%。标记指数最高的是UDA015,为10.80,最低的是UDA001,为6.48,均值为8.68。使用数值分类法和多元分析系统(NTSYS)软件进行聚类分析,采用基于 Jacard 相似矩阵的 UPGMA 方法,将基因型分为 4 个主要聚类。树状图与主成分分析(PCoA)的聚类结果一致。91% 的最高遗传相似性发生在基因型 45 和 51 之间,它们都是同父异母的兄弟和同父异母的杂交后代。结果表明抗幽门螺杆菌的新来源。
更新日期:2020-12-01
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