Abstract Tea beverages have been enjoyed globally for the last several decades. The present study focuses on the comprehensive chemical and biological analysis of three processed tea products. A total of sixty-three compounds in processed samples were identified based on their exact masses and fragmentation patterns using LC-ESI-QTOF-MS/MS. Furthermore, quantification of eight analytes including caffeine (1), theophylline (2), (+)-catechin (3), (-)-epicatechin (4), (-)-epicatechin gallate (5), (-)-gallocatechin (6), (-)-epigallocatechin gallate (7) and quercetin-3- d -β-glucoside (8) in tea samples was performed using LC-ESI-IT-MS/MS. The concentrations of analytes were found in the range of 0.03 mg/g to 75.58 mg/g in all tea samples. The developed method showed excellent accuracy as %bias ranged from 0.2–3.69% and a good precision with %RSD ranged from 0.03 to 5.11. The LOD and LOQ for all the analytes were found to be in the range of 0.16–3.26 ng/mL and 0.44–9.87 ng/mL, respectively. The DPPH scavenging effects of samples were also investigated and all the samples were found to be strong scavengers of DPPH radical showing 69.50 ± 0.01–78.60 ± 0.10 %RSA. The established method provides a useful way for understanding the metabolite distribution in processed products of C. sinensis and to develop quality control protocols for these products.

中文翻译：

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通过 UHPLC-ESI-MS/MS 快速鉴定和量化加工过的山茶样品中的生物活性代谢物并评估其抗氧化活性

摘要 在过去的几十年里，茶饮料在全球范围内广受欢迎。本研究侧重于三种加工茶产品的综合化学和生物分析。使用 LC-ESI-QTOF-MS/MS 根据精确质量数和碎裂模式，共鉴定了处理样品中的 63 种化合物。此外，八种分析物的定量分析，包括咖啡因 (1)、茶碱 (2)、(+)-儿茶素 (3)、(-)-表儿茶素 (4)、(-)-表儿茶素没食子酸酯 (5)、(-)-没食子儿茶素(6)、(-)-表没食子儿茶素没食子酸酯 (7) 和槲皮素-3-d -β-葡糖苷 (8) 使用 LC-ESI-IT-MS/MS 进行分析。在所有茶叶样品中发现分析物的浓度范围为 0.03 mg/g 至 75.58 mg/g。开发的方法显示出极好的准确度，因为 %bias 范围为 0.2-3。69% 和良好的精密度，%RSD 范围为 0.03 至 5.11。发现所有分析物的 LOD 和 LOQ 分别在 0.16–3.26 ng/mL 和 0.44–9.87 ng/mL 的范围内。还研究了样品的 DPPH 清除效果，发现所有样品都是 DPPH 自由基的强清除剂，显示 69.50 ± 0.01–78.60 ± 0.10 %RSA。所建立的方法提供了一种有用的方法来了解 C. sinensis 加工产品中的代谢物分布，并为这些产品制定质量控制协议。