Sirolimus, a lipophilic macrolide, is a well-known immunosuppressant drug used for coating coronary stents and for preventing rejection of kidney transplants in humans. Since Sirolimus is a relatively large molecule with an average mass 914.172 g/mol, size exclusion chromatography (SEC) was employed for exploring its potential for the estimation of Sirolimus content from blood samples. When human blood samples were spiked with known concentrations of Sirolimus, it was observed that it could be estimated with an average recovery of >90% and relative standard deviation <5%. Results indicated that Sirolimus could be estimated with impressive accuracy and repeatability by SEC technique.

西罗莫司是一种亲脂性大环内酯类药物，是一种众所周知的免疫抑制剂，可用于涂覆冠状动脉支架并防止人类肾脏移植排斥。由于西罗莫司是一个相对较大的分子，平均质量为914.172 g / mol，因此采用尺寸排阻色谱（SEC）来探索其从血样中估算西罗莫司含量的潜力。当用已知浓度的西罗莫司加标人体血液样品时，可以观察到其平均回收率> 90％，相对标准偏差<5％。结果表明，通过SEC技术可以估计出西罗莫司的准确性和可重复性。