The detection of biomolecules such as dopamine (DA), ascorbic acid (AA), serotonin (5-HT), and guanine (GU) is essential for biomedical chemistry and disease diagnosis. In this work, MoS₂/acid-treated MWCNTs composite (Ms-atCNTs) was first prepared and then used to modify carbon paste electrode (CPE), followed by electropolymerization of alanine in the presence of NaOH. The resulting modified electrode (p-Aln/Ms-atCNTCPE) was tested for the simultaneous detection of DA, AA, 5-HT, and GU using cyclic voltammetry and differential pulse voltammetry in phosphate buffer solution (PBS). The p-Aln/Ms-atCNTCPE showed excellent electrochemical response towards the detection of DA, AA, 5-HT, and GU. The electrode was also effectively utilized for concentration variation studies of a mixture containing these biomolecules. The detection limits were found to be 0.08 μM, 3.9 μM, 0.1 μM, and 0.1 μM for DA, AA, 5-HT, and GU, respectively. Furthermore, the proposed sensor displayed favorable repeatability, stability, and selectivity, as well as the usefulness for the detection of target molecules in real samples, such as DA injections, vitamin C tablets, herring sperm DNA, and human blood serum.

多巴胺（DA），抗坏血酸（AA），5-羟色胺（5-HT）和鸟嘌呤（GU）等生物分子的检测对于生物医学化学和疾病诊断至关重要。在这项工作中，MoS ₂先制备/酸处理的MWCNTs复合材料（Ms-atCNTs），然后将其用于修饰碳糊电极（CPE），然后在NaOH存在下进行丙氨酸的电聚合。使用循环伏安法和微分脉冲伏安法在磷酸盐缓冲溶液（PBS）中测试所得修饰电极（p-Aln / Ms-atCNTCPE）同时检测DA，AA，5-HT和GU。p-Aln / Ms-atCNTCPE对DA，AA，5-HT和GU的检测显示出出色的电化学响应。该电极还有效地用于含有这些生物分子的混合物的浓度变化研究。发现DA，AA，5-HT和GU的检出限分别为0.08μM，3.9μM，0.1μM和0.1μM。此外，建议的传感器显示出良好的重复性，稳定性和选择性，