Key Message

Two PaGL1-like genes were identified in London plane and functional in Arabidopsis, moreover, may play an important role in the regulation of trichome development in London plane.

Abstract

Trichome development is governed by a complex regulatory network. In Arabidopsis, subgroup 15 of the R2R3 MYB transcription factor family, which includes GLABRA1 (GL1), is involved in trichome development. In this study, we isolated and characterized two PaGL1-like genes from London plane (Platanus acerifolia). Sequence alignment and phylogenetic analysis indicated that these PaGL1-like genes are homologous to AtGL1. Quantitative real-time PCR (qRT-PCR) analysis showed that PaGL1-like1 was expressed in all of the tested organs taken from adult London plane trees, including trichomes, petioles after trichome removal, stems after trichome removal, and leaves after trichome removal, and also in the roots, cotyledons, hypocotyls and true leaves of seedlings. By contrast, the PaGL1-like2 was expressed only in the trichomes and leaves after trichome removal from adult trees, and in the cotyledons and true leaves of seedlings. Overexpression of PaGL1-like genes caused trichome abortion when transferred into wild type Arabidopsis and promoted trichome formation in the gl1 mutant. The expression profiles of some trichome-related genes were changed in transgenic Arabidopsis lines, and yeast two-hybrid analysis indicated that PaGL1-like proteins can directly interact with trichome-related bHLH proteins from both P. acerifolia and Arabidopsis. These results suggest that PaGL1-like genes are functional in Arabidopsis and may play an important role in the regulation of trichome development in London plane.

中文翻译：

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梧桐中与毛状体调控相关的 PaGL1 样基因的鉴定和表征。

关键信息

在伦敦平面中鉴定出两个类PaGL1基因，在拟南芥中具有功能，而且可能在伦敦平面的毛状体发育调控中起重要作用。

抽象的

毛状体发育受复杂的监管网络控制。在拟南芥中，包括GLABRA1 ( GL1 ) 在内的 R2R3 MYB 转录因子家族的第 15 亚组参与了毛状体的发育。在这项研究中，我们从伦敦平面 ( Platanus acerifolia ) 中分离和表征了两个PaGL1 样基因。序列比对和系统发育分析表明这些PaGL1 样基因与AtGL1同源。定量实时 PCR (qRT-PCR) 分析表明PaGL1-like1在取自成年伦敦梧桐树的所有受试器官中表达，包括毛状体、去除毛状体后的叶柄、去除状体后的茎和去除状体后的叶，以及幼苗的根、子叶、下胚轴和真叶。相比之下，PaGL1-like2仅在从成年树去除毛状体后的毛状体和叶子中以及在幼苗的子叶和真叶中表达。当转移到野生型拟南芥中时， PaGL1 样基因的过表达导致毛状体流产，并促进gl1中毛状体的形成突变体。在转基因拟南芥株系中，一些毛状体相关基因的表达谱发生了变化，酵母双杂交分析表明，PaGL1 样蛋白可以直接与来自P. acerifolia和拟南芥的毛状体相关 bHLH 蛋白相互作用。这些结果表明，PaGL1 样基因在拟南芥中具有功能，并且可能在伦敦平面毛状体发育的调控中发挥重要作用。