Key Message

Integrative omics approaches revealed a crosstalk among phytohormones during tuberous root development in cassava.

Abstract

Tuberous root formation is a complex process consisting of phase changes as well as cell division and elongation for radial growth. We performed an integrated analysis to clarify the relationships among metabolites, phytohormones, and gene transcription during tuberous root formation in cassava (Manihot esculenta Crantz). We also confirmed the effects of the auxin (AUX), cytokinin (CK), abscisic acid (ABA), jasmonic acid (JA), gibberellin (GA), brassinosteroid (BR), salicylic acid, and indole-3-acetic acid conjugated with aspartic acid on tuberous root development. An integrated analysis of metabolites and gene expression indicated the expression levels of several genes encoding enzymes involved in starch biosynthesis and sucrose metabolism are up-regulated during tuberous root development, which is consistent with the accumulation of starch, sugar phosphates, and nucleotides. An integrated analysis of phytohormones and gene transcripts revealed a relationship among AUX signaling, CK signaling, and BR signaling, with AUX, CK, and BR inducing tuberous root development. In contrast, ABA and JA inhibited tuberous root development. These phenomena might represent the differences between stem tubers (e.g., potato) and root tubers (e.g., cassava). On the basis of these results, a phytohormonal regulatory model for tuberous root development was constructed. This model may be useful for future phytohormonal studies involving cassava.

中文翻译：

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综合组学方法揭示了木薯块根发育过程中植物激素之间的串扰。

关键信息

综合组学方法揭示了木薯块根发育过程中植物激素之间的串扰。

抽象的

块茎根的形成是一个复杂的过程，包括相变以及细胞分裂和径向生长伸长。我们进行了综合分析，以阐明木薯块根形成过程中代谢物、植物激素和基因转录之间的关系（Manihot esculenta克兰茨）。我们还证实了生长素 (AUX)、细胞分裂素 (CK)、脱落酸 (ABA)、茉莉酸 (JA)、赤霉素 (GA)、油菜素内酯 (BR)、水杨酸和吲哚-3-乙酸结合物的作用。与天冬氨酸对块根发育的影响。代谢物和基因表达的综合分析表明，在块根发育过程中，编码淀粉生物合成和蔗糖代谢酶的几种基因的表达水平上调，这与淀粉、糖磷酸盐和核苷酸的积累一致。对植物激素和基因转录物的综合分析揭示了 AUX 信号、CK 信号和 BR 信号之间的关系，其中 AUX、CK 和 BR 诱导块根发育。相反，ABA和JA抑制块根发育。这些现象可能代表了块茎（例如马铃薯）和块根块茎（例如木薯）之间的差异。在此基础上，构建了块根发育的植物激素调控模型。该模型可能对未来涉及木薯的植物激素研究有用。