This work aimed to verify the effects of adding CO₂ and irradiance to Kappaphycus alvarezii cultivation. Thalli were cultured in vitro for 2 weeks under low irradiance (50 μmol photons m⁻² s⁻¹) with the addition of CO₂ according to the following concentrations: 142× 10³ ppm (1CO₂, 0.1 L min⁻¹), 285 × 10³ ppm (2CO₂, 0.2 L min⁻¹) and 428 × 10³ ppm (3CO₂, 0.3 L min⁻¹), followed by culture under higher irradiance (200 μmol photons m⁻² s⁻¹) for 2 more weeks. Control had no CO₂ addition. Afterwards, growth rate, chlorophyll a and carotenoid content were quantified. Samples were submitted to light microscopy and transmission electron microscopy. Growth rates of different treatments or control between experimental periods showed no significant differences, except for 2CO₂ treatment at the end of each period. Kappaphycus alvarezii cultivated with CO₂ addition showed cell wall thickening and increasing quantity of starch granules, chlorophyll a and carotenoids compared with control. Exposed to high irradiance, control samples showed signs of stress, such as changes in chloroplast, starch granules quantity decrease and total chlorophyll a and carotenoids increase. Samples cultivated with CO₂ showed an increase in the quantity and size of the starch granules and an increase in the number of organelles (mitochondria) related to energy generation and cell construction (Golgi complex). Such alterations suggest cellular response after CO₂ addition, such as the formation of starch reserves and cell wall thickening, which could make plants more tolerant to environmental stress during transport from indoor condition to sea farms.

这项工作旨在验证添加二氧化碳₂和辐照度对Kappaphycus alvarezii培养的影响。菌体在体外培养低辐照下2周（50微摩尔光子米^-2小号^-1与添加Co）₂根据下列浓度：142×10 ³  ppm的（1CO ₂，0.1升分钟^-1）， 285×10 ³  ppm的（2CO ₂，0.2升分钟^-1）和428×10 ³  ppm的（3CO ₂，0.3升分钟^-1），然后培养更高的辐照度下（200微摩尔光子米^-2小号^-1）再等待2周。对照没有添加CO ₂。之后，对生长速率，叶绿素a和类胡萝卜素含量进行定量。将样品进行光学显微镜和透射电子显微镜。在实验期之间，不同处理或对照的生长速率无显着差异，除了在每个周期结束时进行2CO ₂处理外。添加了CO _2的Kappaphycus alvarezii表现出细胞壁增厚和淀粉颗粒，叶绿素a数量增加和类胡萝卜素与对照相比。暴露于高辐照度下，对照样品显示出压力迹象，例如叶绿体变化，淀粉颗粒数量减少以及总叶绿素a和类胡萝卜素增加。用CO ₂培养的样品显示出与能量产生和细胞构建（高尔基复合体）相关的淀粉颗粒的数量和大小增加，细胞器（线粒体）数量增加。这种变化表明添加CO ₂后的细胞反应，例如淀粉储备的形成和细胞壁增厚，这可能使植物在从室内条件转移到海洋养殖场的过程中更能耐受环境胁迫。