The objective of this study was to reduce the negative effects of oxidative stress by decreasing the levels of reactive oxygen species (ROS) through supplementation of the major antioxidants present in elderberries: kuromanin and cyanidin. Oocytes (n = 1150) were supplemented with 100 or 200 μM of kuromanin or cyanidin during maturation, and then evaluated for ROS levels or fertilized and evaluated for penetration, polyspermic penetration, male pronucleus formation, and embryonic development. The ROS levels and incidence of polyspermic penetration were lower (P < 0.05) in oocytes supplemented with 100 μM cyanidin when compared with other treatments. Supplementation of 100 μM cyanidin increased (P < 0.05) MPN and blastocyst formation compared with other treatments. However, supplementation of 100 μM kuromanin did not have significant effects on the criteria evaluated, and supplementation of 200 μM kuromanin had significant (P < 0.05) detrimental effects for each criterion. Additional oocytes (n = 1438) were supplemented with 100 μM cyanidin during maturation and evaluated for glutathione, glutathione peroxidase, catalase, and superoxide dismutase activity. Supplementation of 100 μM cyanidin increased (P < 0.05) catalase activity and intracellular GSH levels compared with no supplementation of cyanidin. These results indicate that supplementing cyanidin during maturation reduces oxidative stress by reducing ROS levels and increasing GSH concentrations within the oocyte.

这项研究的目的是通过补充接骨木浆果中存在的主要抗氧化剂：黑素和花青素来降低活性氧（ROS）的水平，从而减少氧化应激的负面影响。卵母细胞（n  = 1150）在成熟过程中补充有100或200μM的kuromanin或花青素，然后评估ROS的水平或受精，并评估其渗透性，多精子渗透性，雄性前核形成和胚胎发育。 与其他治疗方法相比，在补充有100μM矢车菊苷的卵母细胞中，ROS水平和多精子穿透的发生率更低（P <0.05）。增加100μM的花青素（P <0.05）与其他治疗相比，MPN和囊胚形成。但是，补充100μM的Kuromanin对所评估的标准无显着影响，补充200μM的Kuromanin 对每个标准均具有显着（P <0.05）有害作用。 在成熟过程中，向其他卵母细胞（n = 1438）补充了100μM的花青素，并评估了谷胱甘肽，谷胱甘肽过氧化物酶，过氧化氢酶和超氧化物歧化酶的活性。增加100μM的花青素（P <0.05）过氧化氢酶活性和细胞内GSH水平与未添加花青素相比。这些结果表明，在成熟过程中补充花青素可通过降低ROS水平和增加卵母细胞内GSH的浓度来降低氧化应激。