The initial biomass density had a significant effect on the growth behaviour of Leptolyngbya foveolarum HNBGU-001 in the batch culture. The test organism produced substantial amounts of biomass and lipids when allowed to grow at 40 °C and pH 8.0. The Placket-Burman design appropriately indicated the positive and negative influences of the tested nutrient factors on biomass and lipid productivities of L. foveolarum. Employing the Box-Behnken design of response surface methodology, the elevated concentrations of sulphate-S (300 mg/L) and carbonate-C (45.39 mg/L) but relatively low levels of phosphate-P (10 mg/L) and nitrate-N (375 mg/L) were identified as the optimal nutritional requirements of the test organism for the maximum lipid productivity (49.60 ± 0.70 mg/L/day) along with high lipid content (32.10 ± 0.40% dry cell weight) and biomass productivity (154.80 ± 3.60 mg/L/day). These maximized responses of biomass productivity, lipid content, and lipid productivity were respectively 2.8-, 2.4-, and 6.8-fold higher than their corresponding values under the unoptimized conditions. FAME analysis of lipids obtained from the test organism revealed some promising features for their use as algal biodiesel feedstock. Nonetheless, the test organism seems to have better potential than several other microalgae for use in biofuel production as it can give a high output of biomass as also lipids at elevated temperature prevalent in outdoor conditions of subtropical and tropical regions.

在分批培养中，初始生物量密度对黄斑镰刀菌HNBGU-001的生长行为具有显着影响。当在40°C和pH 8.0下生长时，测试生物产生了大量生物质和脂质。Placket-Burman设计适当地表明了所测试的营养因子对黄腐乳杆菌生物量和脂质生产率的正负影响。采用响应表面方法的Box-Behnken设计，可以提高硫酸盐S（300 mg / L）和碳酸盐C（45.39 mg / L）的浓度，但磷酸盐P（10 mg / L）和硝酸盐的水平相对较低-N（375 mg / L）被确定为测试有机体最大脂质生产率（49.60±0.70 mg / L / day）以及高脂质含量（32.10±0.40％干细胞重量）和生物质的最佳营养需求生产率（154.80±3.60 mg / L /天）。在未优化的条件下，生物量生产力，脂质含量和脂质生产力的这些最大响应分别比其相应值高2.8倍，2.4倍和6.8倍。对从测试生物中获得的脂质进行的FAME分析表明，将其用作藻类生物柴油原料具有一些很有前途的特征。尽管如此，