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Interaction of nano-quantum dots (CdSe@ZnS) and extracellular proteins in activated sludge revealed by bio-nano science
Environmental Science: Nano ( IF 5.8 ) Pub Date : 2020-08-04 , DOI: 10.1039/d0en00392a Aibin Hu 1, 2, 3, 4 , Weijun Zhang 1, 2, 3, 4 , Guiying Liao 2, 3, 4, 5, 6 , Dongsheng Wang 1, 2, 3, 4, 7 , Yongzhen Peng 4, 8, 9, 10, 11
Environmental Science: Nano ( IF 5.8 ) Pub Date : 2020-08-04 , DOI: 10.1039/d0en00392a Aibin Hu 1, 2, 3, 4 , Weijun Zhang 1, 2, 3, 4 , Guiying Liao 2, 3, 4, 5, 6 , Dongsheng Wang 1, 2, 3, 4, 7 , Yongzhen Peng 4, 8, 9, 10, 11
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The protein corona has gained much attention in the field of nano-biomedicine and has expanded from applications in the biological field to the ecosphere. However, the bio-corona (extracellular protein corona) composed of nano-quantum dots (CdSe@ZnS) and extracellular proteins in an activated sludge system was limited by the inability to identify the composition and molecular structure of extracellular proteins. Herein, extracellular proteins (EP) were extracted from the active sludge (AS) system and purified by using a novel method (modified-ammonium sulfate precipitate) and then identified by proteomic technology. The interaction between nano-quantum dots and extracellular protein was investigated with multi-spectroscopic technology and microscopic analysis. The results revealed that 97 protein groups were detected and most of their molecular functions were related to the cation binding activity. The self-aggregation effect of protein particles and the formation of the bio-corona were observed. Meanwhile, α-helical structures of EPs in active sludge were converted into β-sheets conformation via electrostatic and hydrogen bonding processes, with the addition of nano-quantum dots, resulting in decreased fluorescence intensities for proteins. This finding not only provided a novel method for the purification of EP from extracellular polymeric substances, but also provided an insight into the interaction between NPs and actual EP in the AS system.
中文翻译：
生物纳米科学揭示了活性污泥中纳米量子点（CdSe @ ZnS）与细胞外蛋白的相互作用
电晕蛋白已在纳米生物医学领域引起了广泛关注，并已从生物领域的应用扩展到生态圈。然而，由活性污泥系统中的纳米量子点（CdSe @ ZnS）和细胞外蛋白质组成的生物电晕（细胞外蛋白质电晕）受到无法识别细胞外蛋白质的组成和分子结构的限制。本文中，从活性污泥（AS）系统中提取细胞外蛋白（EP），并使用新方法（改性硫酸铵沉淀）纯化，然后通过蛋白质组学技术进行鉴定。利用多光谱技术和显微分析技术研究了纳米量子点与细胞外蛋白之间的相互作用。结果表明，检测到97个蛋白质基团，其大多数分子功能与阳离子结合活性有关。观察到蛋白质颗粒的自聚集效应和生物电晕的形成。同时，活性污泥中EPs的α-螺旋结构转化为β-sheets构象。通过静电和氢键工艺，加上纳米量子点，导致蛋白质的荧光强度降低。该发现不仅提供了从细胞外聚合物中纯化EP的新颖方法，而且还提供了对AS系统中NP与实际EP之间相互作用的了解。
更新日期：2020-09-18
中文翻译：
生物纳米科学揭示了活性污泥中纳米量子点（CdSe @ ZnS）与细胞外蛋白的相互作用
电晕蛋白已在纳米生物医学领域引起了广泛关注，并已从生物领域的应用扩展到生态圈。然而，由活性污泥系统中的纳米量子点（CdSe @ ZnS）和细胞外蛋白质组成的生物电晕（细胞外蛋白质电晕）受到无法识别细胞外蛋白质的组成和分子结构的限制。本文中，从活性污泥（AS）系统中提取细胞外蛋白（EP），并使用新方法（改性硫酸铵沉淀）纯化，然后通过蛋白质组学技术进行鉴定。利用多光谱技术和显微分析技术研究了纳米量子点与细胞外蛋白之间的相互作用。结果表明，检测到97个蛋白质基团，其大多数分子功能与阳离子结合活性有关。观察到蛋白质颗粒的自聚集效应和生物电晕的形成。同时，活性污泥中EPs的α-螺旋结构转化为β-sheets构象。通过静电和氢键工艺，加上纳米量子点，导致蛋白质的荧光强度降低。该发现不仅提供了从细胞外聚合物中纯化EP的新颖方法，而且还提供了对AS系统中NP与实际EP之间相互作用的了解。
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