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Development and application of ubiquitin-based chemical probes
Chemical Science ( IF 7.6 ) Pub Date : 2020-08-04 , DOI: 10.1039/d0sc03295f Xin Sui 1, 2, 3, 4, 5 , Yu Wang 1, 2, 3, 4, 5 , Yun-Xiang Du 6, 7, 8, 9, 10 , Lu-Jun Liang 6, 7, 8, 9, 10 , Qingyun Zheng 6, 7, 8, 9, 10 , Yi-Ming Li 1, 2, 3, 4, 5 , Lei Liu 6, 7, 8, 9, 10
Chemical Science ( IF 7.6 ) Pub Date : 2020-08-04 , DOI: 10.1039/d0sc03295f Xin Sui 1, 2, 3, 4, 5 , Yu Wang 1, 2, 3, 4, 5 , Yun-Xiang Du 6, 7, 8, 9, 10 , Lu-Jun Liang 6, 7, 8, 9, 10 , Qingyun Zheng 6, 7, 8, 9, 10 , Yi-Ming Li 1, 2, 3, 4, 5 , Lei Liu 6, 7, 8, 9, 10
Affiliation
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Protein ubiquitination regulates almost every process in eukaryotic cells. The study of the many enzymes involved in the ubiquitination system and the development of ubiquitination-associated therapeutics are important areas of current research. Synthetic tools such as ubiquitin-based chemical probes have been making an increasing contribution to deciphering various biochemical components involved in ubiquitin conjugation, recruitment, signaling, and deconjugation. In the present minireview, we summarize the progress of ubiquitin-based chemical probes with an emphasis on their various structures and chemical synthesis. We discuss the utility of the ubiquitin-based chemical probes for discovering and profiling ubiquitin-dependent signaling systems, as well as the monitoring and visualization of ubiquitin-related enzymatic machinery. We also show how the probes can serve to elucidate the molecular mechanism of recognition and catalysis. Collectively, the development and application of ubiquitin-based chemical probes emphasizes the importance and utility of chemical protein synthesis in modern chemical biology.
中文翻译：
基于泛素的化学探针的开发与应用
蛋白质泛素化调节真核细胞中的几乎每个过程。泛素化系统中涉及的许多酶的研究以及泛素化相关疗法的发展是当前研究的重要领域。诸如基于遍在蛋白的化学探针之类的合成工具对破译涉及遍在蛋白缀合，募集，信号传导和去缀合的各种生化成分做出了越来越大的贡献。在本篇综述中，我们总结了基于泛素的化学探针的研究进展，重点是其各种结构和化学合成。我们讨论了基于泛素的化学探针对发现和分析泛素依赖性信号传导系统以及泛素相关酶机制的监测和可视化的实用性。我们还展示了探针如何可用于阐明识别和催化的分子机制。总的来说，基于泛素的化学探针的开发和应用强调了化学蛋白质合成在现代化学生物学中的重要性和实用性。
更新日期：2020-08-04
中文翻译：
基于泛素的化学探针的开发与应用
蛋白质泛素化调节真核细胞中的几乎每个过程。泛素化系统中涉及的许多酶的研究以及泛素化相关疗法的发展是当前研究的重要领域。诸如基于遍在蛋白的化学探针之类的合成工具对破译涉及遍在蛋白缀合，募集，信号传导和去缀合的各种生化成分做出了越来越大的贡献。在本篇综述中，我们总结了基于泛素的化学探针的研究进展，重点是其各种结构和化学合成。我们讨论了基于泛素的化学探针对发现和分析泛素依赖性信号传导系统以及泛素相关酶机制的监测和可视化的实用性。我们还展示了探针如何可用于阐明识别和催化的分子机制。总的来说，基于泛素的化学探针的开发和应用强调了化学蛋白质合成在现代化学生物学中的重要性和实用性。
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