Objective:

We examined the immunohistochemical expression of α-methyl acyl coenzyme A racemase (AMACR), CD10, TMPRSS2-ERG, and p27 in prostate adenocarcinoma tumors with different Gleason growth patterns and nonneoplastic prostate tissues to elucidate their roles in prostate carcinogenesis and histological aggressiveness.

Material and Methods:

In total, 80 archival core biopsy tissues diagnosed as prostate carcinoma, benign prostate hyperplasia, and atrophy cases were included. Immunoreactivity was evaluated by calculating the percentage of positively stained cells and the staining intensity. The mean values and test of significance were obtained using the Kruskal-Wallis test.

Results:

We obtained mostly intense immunoreactivity for AMACR, CD10, and ERG in adenocarcinomas. Although no significant differences were noted regarding AMACR and ERG expression, samples with Gleason growth patterns 3 and 5 tended to be strongly positive for ERG. Pattern 3 tumors exhibited the weakest positivity for CD10. The p27 expression was strong and diffuse in nonneoplastic prostate tissues. The loss of p27 expression was more frequent for pattern 5 tumors.

Conclusion:

ERG and AMACR were powerful markers to detect cancer. Especially, ERG is evident in early tumors may reflect its interaction with functional androgen receptors in cancer initiation. Pattern 5 tumors associated with stroma may have been exposed to more stromal substrates and upregulate their CD10 content as a protein degrader. We suggest that CD10 expression is associated with an increasing tumor grade. Decreased concentrations of p27 protein might be implicated in prostate carcinogenesis and may be a useful immunohistochemical adjunct in predicting histological aggressiveness.

中文翻译：

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AMACR、CD10、TMPRSS2-ERG 和 p27 蛋白表达在不同 Gleason 分级前列腺癌中对针刺活检的作用

客观的：

我们检测了 α-甲基酰基辅酶 A 消旋酶 (AMACR)、CD10、TMPRSS2-ERG 和 p27 在具有不同 Gleason 生长模式的前列腺癌肿瘤和非肿瘤性前列腺组织中的免疫组织化学表达，以阐明它们在前列腺癌发生和组织学侵袭性中的作用。

材料与方法：

总共包括 80 个被诊断为前列腺癌、良性前列腺增生和萎缩病例的档案核心活检组织。通过计算阳性染色细胞的百分比和染色强度来评估免疫反应性。使用 Kruskal-Wallis 检验获得平均值和显着性检验。

结果：

我们在腺癌中获得了 AMACR、CD10 和 ERG 的大部分强烈免疫反应性。尽管 AMACR 和 ERG 表达没有显着差异，但 Gleason 生长模式 3 和 5 的样本往往对 ERG 呈强阳性。模式 3 肿瘤对 CD10 表现出最弱的阳性。p27 表达在非肿瘤性前列腺组织中强烈且弥漫。对于模式 5 肿瘤，p27 表达的丧失更为常见。

结论：

ERG 和 AMACR 是检测癌症的有力标志物。特别是，ERG 在早期肿瘤中很明显，这可能反映了它在癌症发生过程中与功能性雄激素受体的相互作用。与基质相关的 5 型肿瘤可能已暴露于更多基质底物，并作为蛋白质降解剂上调其 CD10 含量。我们认为 CD10 表达与肿瘤分级增加有关。p27 蛋白浓度的降低可能与前列腺癌发生有关，并且可能是预测组织学侵袭性的有用的免疫组织化学辅助手段。