Many foodstuffs are extremely susceptible to contamination with aflatoxins, in which aflatoxin B1 is highly toxic and carcinogenic. Therefore, it is crucial to develop a rapid and effective analytical method for detecting and monitoring aflatoxin B1 in food. Herein, a surface-enhanced Raman spectroscopic (SERS) method combined with QuEChERS (quick, easy, cheap-effective, rugged, safe) sample pretreatment technique was used to detect aflatoxin B1. Sample preparation was optimized into a one-step extraction method using an Au nanoparticle-based solution (Au sol) as the SERS detection substrate. An affordable portable Raman spectrometer was then used for rapid, label-free, quantitative detection of aflatoxin B1 levels in foodstuffs. This method showed a good linear log relationship between the Raman signal intensity of aflatoxin B1 in the 1–1000 µg L–1 concentration range with a limit of detection of 0.85 µg kg–1 and a correlation coefficient of 0.9836. Rapid aflatoxin B1 detection times of ∼10 min for wheat, corn, and protein feed powder samples were also achieved. This method has high sensitivity, strong specificity, excellent stability, is simple to use, economical, and is suitable for on-site detection, with good prospects for practical application in the field of food safety.

中文翻译：

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EXPRESS：使用便携式拉曼光谱仪快速定量检测谷物中的黄曲霉毒素 B1

许多食品极易受到黄曲霉毒素的污染，其中黄曲霉毒素 B1 具有剧毒和致癌性。因此，开发一种快速有效的分析方法来检测和监测食品中的黄曲霉毒素B1至关重要。在此，采用表面增强拉曼光谱 (SERS) 方法结合 QuEChERS（快速、简便、廉价、坚固、安全）样品前处理技术检测黄曲霉毒素 B1。样品制备被优化为一步提取方法，使用基于 Au 纳米颗粒的溶液 (Au sol) 作为 SERS 检测底物。然后使用经济实惠的便携式拉曼光谱仪快速、无标记地定量检测食品中的黄曲霉毒素 B1 水平。该方法表明，黄曲霉毒素 B1 的拉曼信号强度在 1–1000 µg L–1 浓度范围内具有良好的线性对数关系，检测限为 0.85 µg kg–1，相关系数为 0.9836。还实现了小麦、玉米和蛋白质饲料粉末样品的黄曲霉毒素 B1 快速检测时间约为 10 分钟。该方法灵敏度高、特异性强、稳定性好、使用简单、经济、适合现场检测，在食品安全领域具有良好的实际应用前景。