Objective

Osteoarthritis (OA) commonly affects weight-bearing joints and is characterized by articular cartilage breakdown combined with osteophyte formation at the joint margins and chronic nonspecific inflammation of synovium. Understanding the profile of inflammation in a patient population is an essential starting point to predict or prevent OA progression. The aim of this study was to identify the profile of selected biomolecules in synovial fluid (SF) and investigate the correlation according to gender, age, and severity of the disease within patients from among the general knee OA population.

Design

In our study SF samples were aspirated from the knees of 65 OA patients (46 patients with early knee OA and 19 patients with end-stage knee OA according to the Kellgren-Lawrence grading scale). The concentration of interleukins (IL-6, IL-8), matrix metalloproteinases (MMP-1, MMP-3, MMP-13), MMPs inhibitors (TIMP-1, TIMP-2), cartilage oligomeric matrix protein (COMP), and adiponectin was analyzed using a multiplex ELISA-based approach.

Conclusions

Our results indicate significant linear correlation of MMP-13 and COMP concentration with age (P < 0.05), but not with OA severity. In fact, 3 of the examined biomolecules, MMP-3 (P < 0.01), TIMP-1 (P < 0.01), and COMP (P < 0.05) significantly correlate with the grade of knee OA and might be associated with OA severity.

中文翻译：

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联合检测滑液中 COMP、TIMP-1 和 MMP-3 的初步研究：骨关节炎进展的潜在指标。

客观的

骨关节炎（OA）通常影响负重关节，其特征是关节软骨破裂，关节边缘骨赘形成和滑膜慢性非特异性炎症。了解患者群体的炎症特征是预测或预防 OA 进展的重要起点。本研究的目的是确定滑液 (SF) 中选定生物分子的特征，并根据一般膝关节 OA 人群中患者的性别、年龄和疾病严重程度调查相关性。

设计

在我们的研究中，SF 样本是从 65 名 OA 患者（根据 Kellgren-Lawrence 分级量表，46 名早期膝 OA 患者和 19 名终末期膝 OA 患者）的膝部抽取的。白细胞介素（IL-6、IL-8）、基质金属蛋白酶（MMP-1、MMP-3、MMP-13）、MMPs抑制剂（TIMP-1、TIMP-2）、软骨寡聚基质蛋白（COMP）的浓度，使用基于多重ELISA的方法分析脂联素。

结论

我们的结果表明 MMP-13 和 COMP 浓度与年龄显着线性相关（P < 0.05），但与 OA 严重程度无关。事实上，检查的 3 个生物分子 MMP-3 ( P < 0.01)、TIMP-1 ( P < 0.01) 和 COMP ( P < 0.05) 与膝关节 OA 的等级显着相关，并可能与 OA 严重程度相关。