Collecting large numbers of rare cells for high-throughput molecular analysis remains a technical challenge, primarily due to limitations in existing technologies. In developmental biology this has impeded single-cell analysis of primordial organs, which derive from few cells. In this study, we share novel transgenic lines for rapid cell enrichment from zebrafish embryos using human surface antigens for immunological binding and magnetic sorting. As proof of principle, we tagged, enriched, and performed single-cell RNA sequencing on nascent hematopoietic stem/progenitor cells and endothelial cells from early embryos. Our method is a quick, efficient, and cost-effective approach to a previously intractable problem.

中文翻译：

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一种用于快速富集稀有胚胎细胞的转基因系统。

收集大量稀有细胞用于高通量分子分析仍然是一项技术挑战，主要是由于现有技术的局限性。在发育生物学中，这阻碍了对源自少数细胞的原始器官的单细胞分析。在这项研究中，我们分享了使用人类表面抗原进行免疫结合和磁性分选的斑马鱼胚胎快速细胞富集的新型转基因品系。作为原理证明，我们对来自早期胚胎的新生造血干/祖细胞和内皮细胞进行了标记、富集和单细胞 RNA 测序。我们的方法是解决以前难以解决的问题的一种快速、高效且具有成本效益的方法。