The sugars will eventually be exported transporters (SWEET) gene family is a glycoprotein gene family that can regulate the transport of sugar in plants and plays an important role in plant growth and development, as well as in response to environmental stress. In this study, Kentucky bluegrass (cv. Baron) seedlings were grown in various treatments, including heavy metal cadmium, salt, drought, cold, and heat stress for 6 h, 24 h, 48 h, and 7 day. The relative expression of the identified PpSWEET genes in Kentucky bluegrass was measured. The results showed there were a total of 13 SWEET genes, which could be divided into four clades by phylogenetic analysis. Most PpSWEET genes are alkali proteins with seven transmembrane helices. Moreover, almost all PpSWEET proteins possess similar conserved motifs and active sites. In addition, an analysis of the relative expression of PpSWEET genes under various stress treatments indicated that PpSWEET12 and PpSWEET15 had very high expression under the five types of stress, meaning they can be used as important candidate genes for studying responses to environmental stresses of turfgrass. Furthermore, certain genes only showed changes in expression under one or two specific stress treatments. This study provides important insight into the SWEET gene family in Kentucky bluegrass and its functional roles in responses to various environmental stresses.

中文翻译：

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非生物胁迫下草地早熟禾SWEET基因家族的鉴定与表达分析。

糖最终将被出口转运蛋白（SWEET）基因家族，是一种糖蛋白基因家族，可以调节植物中糖的运输，并在植物生长和发育以及应对环境胁迫中发挥重要作用。在这项研究中，肯塔基蓝草（cv。Baron）幼苗通过多种处理方法生长，包括重金属镉，盐，干旱，寒冷和高温胁迫，分别处理6小时，24小时，48小时和7天。测量已鉴定的PpSWEET基因在肯塔基州蓝草中的相对表达。结果表明，共有13个SWEET基因，通过系统发育分析可以分为四个进化枝。大多数PpSWEET基因是具有七个跨膜螺旋的碱性蛋白。此外，几乎所有的PpSWEET蛋白都具有相似的保守基序和活性位点。此外，对各种胁迫处理下PpSWEET基因相对表达的分析表明，PpSWEET12和PpSWEET15在五种胁迫下均具有很高的表达，这意味着它们可以用作研究草皮草对环境胁迫反应的重要候选基因。此外，某些基因仅在一种或两种特定的应激处理下显示出表达变化。这项研究为肯塔基州蓝草的SWEET基因家族及其在应对各种环境胁迫中的功能作用提供了重要的见识。