Plants circumscribe microbe-associated molecular pattern (MAMP)-triggered immune responses to weak points of the roots. This spatially restricted immunity was suggested to avoid constitutive responses to rhizosphere microbiota. To demonstrate its relevance, we combined cell-type specific expression of the plant flagellin receptor (FLS2) with fluorescent defence markers and mapped immune competency at cellular resolution. Our analysis distinguishes cell-autonomous and non-cell autonomous responses and reveals lignification to be tissue-independent, contrasting cell-type specific suberisation. Importantly, our analysis divides the non-responsive meristem into a central zone refractory to FLS2 expression, and a cortex that becomes highly sensitised by FLS2 expression, causing meristem collapse upon MAMP exposure. Meristematic epidermal expression generates supercompetent lines that detect native bacterial flagellin and bypass the absence of response to commensals, providing a powerful tool for studying root immunity. Our precise manipulations and read-outs demonstrate incompatibility of meristematic activity and defence and the importance of cell-resolved studies of plant immunity.

中文翻译：

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在细菌定植过程中，空间受限的免疫反应可引起根性分生组织活动。

植物限制了微生物相关分子模式（MAMP）触发的对根薄弱点的免疫反应。建议在空间上限制免疫力以避免对根际微生物群的组成性反应。为了证明其相关性，我们将植物鞭毛蛋白受体（FLS2）的细胞类型特异性表达与荧光防御标记相结合，并在细胞分辨率下绘制了免疫能力。我们的分析区分了细胞自主反应和非细胞自主反应，并揭示了木质素化是组织独立的，与细胞类型特异的干物质形成了对比。重要的是，我们的分析将非反应性分生组织分为难于FLS2表达的中央区域和皮质，该皮质被FLS2表达高度敏感，导致暴露于MAMP的分生组织崩溃。分生组织的表皮表达产生能检测天然细菌鞭毛蛋白的超能力品系，并绕过对共价反应的不存在，为研究根的免疫力提供了强大的工具。我们的精确操作和读数表明，分生组织活动和防御能力不兼容，并且无法通过细胞解析研究植物免疫力。