Background: A diet rich in cruciferous vegetables is reported to have beneficial health effects, partially mediated by 4-methylsulfinylbutyl glucosinolate, or glucoraphanin, which is predominantly found within broccoli (Brassica oleracea var italica). We describe the downstream effects on transcription and metabolism in broccoli following the introgression of a genetic variant of MYB28 into broccoli from a wild Brassica relative which has previously been associated with enhancement of glucoraphanin. Results: Whole genome sequencing, RNA expression and metabolite analyses were used to characterise the consequences of the introgression of either one or two copies of a genetic variant of the MYB28 transcription factor into a commercial broccoli genetic background. The introgression of the variant of MYB28 resulted in enhanced expression of genes involved in primary sulphate assimilation, sulphur metabolism and aliphatic glucosinolate biosynthesis, and enhanced accumulation of 4-methylsulphinyl butyl glucosinolate in florets. Other changes in transcription that may be related to non-targeted introgression events are reported. There were no consistent effects upon sulphur metabolites pools, apart from methionine-derived glucosinolates. Conclusion: This study illustrates the downstream effects on transcription and metabolism of the introgression of a genetic variant of MYB28 from a wild species into a commercial broccoli genotype.

中文翻译：

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R2R3 MYB转录因子渗入对甘蓝型油菜硫代谢的影响

背景：据报道，富含十字花科蔬菜的饮食具有有益的健康效果，部分由4-甲基亚磺酰基丁基芥子油苷或葡糖甘露聚糖介导，后者主要存在于西兰花（芸苔）中。我们描述了MYB28的遗传变异从野生芸苔属相对于花椰菜渗入花椰菜后，下游对花椰菜转录和代谢的影响，该花青素以前与葡糖甘蓝的增强有关。结果：使用全基因组测序，RNA表达和代谢物分析来表征将一或两个拷贝的MYB28转录因子遗传变异体渗入商业花椰菜遗传背景的后果。MYB28变体的渗入导致参与初级硫酸盐同化，硫代谢和脂肪族芥子油苷生物合成的基因的表达增强，以及4-小亚甲基丁基芥子油苷在小花中的积累。据报道可能与非靶向渗入事件有关的其他转录变化。除蛋氨酸衍生的硫代葡萄糖苷外，对硫代谢产物库没有一致的影响。结论：这项研究说明了MYB28基因变体从野生物种向商业花椰菜基因型基因渗入对转录和代谢的下游影响。据报道可能与非靶向渗入事件有关的其他转录变化。除蛋氨酸衍生的芥子油苷外，对硫代谢物库没有持续的影响。结论：这项研究说明了MYB28基因变体从野生物种向商业花椰菜基因型基因渗入对转录和代谢的下游影响。据报道可能与非靶向渗入事件有关的其他转录变化。除蛋氨酸衍生的芥子油苷外，对硫代谢物库没有持续的影响。结论：这项研究说明了MYB28基因变体从野生物种向商业花椰菜基因型基因渗入对转录和代谢的下游影响。