Candidatus Liberibacter asiaticus (CLas) has been associated with Huanglongbing, a lethal vector-borne disease affecting citrus crops worldwide. While comparative genomics has provided preliminary insights into the metabolic capabilities of this uncultured microorganism, a comprehensive functional characterization is currently lacking. Here, we reconstructed and manually curated genome-scale metabolic models for the six CLas strains A4, FL17, gxpsy, Ishi-1, psy62, and YCPsy, in addition to a model of the closest related culturable microorganism, L. crescens BT-1. Predictions about nutrient requirements and changes in growth phenotypes of CLas were confirmed using in vitro hairy root-based assays, while the L. crescens BT-1 model was validated using cultivation assays. Host-dependent metabolic phenotypes were revealed using expression data obtained from CLas-infected citrus trees and from the CLas-harboring psyllid Diaphorina citri Kuwayama. These results identified conserved and unique metabolic traits, as well as strain-specific interactions between CLas and its hosts, laying the foundation for the development of model-driven Huanglongbing management strategies.

Candidatus Liberibacter asiaticus ( C Las) 与黄龙病有关，黄龙病是一种影响全世界柑橘作物的致命媒介传播疾病。虽然比较基因组学已经为这种未培养微生物的代谢能力提供了初步见解，但目前缺乏全面的功能表征。在这里，我们重建并手动策划了六种C Las 菌株 A4、FL17、gxpsy、Ishi-1、psy62 和 YCPsy 的基因组规模代谢模型，以及最接近的可培养微生物L. crescens BT 的模型。 1.使用体外基于毛状根的测定证实了对C Las 营养需求和生长表型变化的预测，同时使用培养测定验证了L. crescens BT-1 模型。使用从感染C Las 的柑橘树和携带C Las 木虱Diaphorina citri Kuwayama 获得的表达数据揭示了宿主依赖性代谢表型。这些结果确定了保守且独特的代谢特征，以及C Las 与其宿主之间的菌株特异性相互作用，为模型驱动的黄龙病管理策略的开发奠定了基础。