Abstract

Fabricating scaffolds with biomimetic architectures is an important step toward engineering functional tissues. Electrospinning is a popular approach for creating nanofibrous substrates in which the filaments resembling the natural extracellular matrix (ECM) can provide topographical cues to cells directing their growth. One of the major challenges in electrospinning is tailoring the spatial organization of the filaments. To overcome this challenge, a hybrid static collector was utilized to form distinct filament organizations. The filament organization was characterized using image processing based on the Fourier Transforms Method. The effect of different filament orientation ratios on cellular growth is discussed.

摘要

制造具有仿生结构的支架是实现功能组织工程化的重要一步。静电纺丝是一种流行的制造纳米纤维基材的方法，其中类似于天然细胞外基质 (ECM) 的细丝可以为指导其生长的细胞提供地形线索。静电纺丝的主要挑战之一是定制细丝的空间组织。为了克服这一挑战，使用混合静态收集器来形成不同的细丝组织。使用基于傅立叶变换方法的图像处理来表征细丝组织。讨论了不同长丝取向比对细胞生长的影响。