Abstract

Eight compounds were obtained from the dry fruits of Piper longum L., and their potential vascular relaxant activities were explored. The present study first revealed the access of Rosin (7) and Piperchabaoside (8) in the medicinal plant Piper longum L. The vessel tension studies showed that Piperine (2), (2E,4E,14Z)-N-isobutyleicosa-2,4,14-trienamide (3), and Piperlonguminine (6) exerted significant inhibitory effects on PE-induced mesenteric artery vasoconstriction. Furthermore, Calcium Imaging studies were applied to observe the effect of Piperine on the intracellular calcium in mesenteric artery smooth muscle cells (MASMCs). Piperine (2) was observed to promote the influx of extracellular calcium in MASMCs, and via an endothelium-independent mechanism involving Ca²⁺ entry. Piper longum L. might have a great potential to be further studied as a vascular relaxant, even to be a drug candidate of anti-hypertension.

摘要

从Piper longum L.的干果中提取了八种化合物，并探索了它们潜在的血管松弛活性。本研究首次揭示了药用植物Piper longum L 中松香 ( 7 ) 和 Piperchabaoside ( 8 ) 的通路。血管张力研究表明 Piperine ( 2 ), (2E,4E,14Z)-N-isobutyleicosa-2, 4,14-三烯酰胺 ( 3 ) 和 Piperlonguminine ( 6 ) 对 PE 引起的肠系膜动脉血管收缩具有显着的抑制作用。此外，钙成像研究用于观察胡椒碱对肠系膜动脉平滑肌细胞 (MASMCs) 细胞内钙的影响。胡椒碱 ( 2) 被观察到促进 MASMC 中细胞外钙的流入，并通过涉及 Ca ²⁺进入的内皮非依赖性机制。Piper longum L. 可能具有作为血管松弛剂进一步研究的巨大潜力，甚至可能成为抗高血压的候选药物。