ABSTRACT Praziquantel (PZQ) is a compound that usually used as insecticide, drug residue could be harm to human health. In this study, we have developed a simple, sensitive, and rapid lateral flow immunochromatographic assay (LFIA) for the detection of PZQ in fish (Lateolabrax Japonicus). We produced a sensitive mAb using enzyme-linked immunosorbent assay with half-maximal inhibitory concentration (2A11, IC50) of 2.06 ng/mL. The coating antigen (PZQ-HS-GA-OVA) and goat anti-mouse IgG antibody were sprayed onto a nitrocellulose membrane as the test and control lines, respectively. Under optimal conditions, the cut-off limits for PZQ detection on the test strips were determined to be 5 ng/mL in both 0.01 M phosphate buffered saline and fish samples, and results were obtained within 5 min. Our data suggests that this LFIA can be used for the sensitive, rapid, and specific on-site screening for PZQ in fish.

中文翻译：

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使用侧流免疫色谱法超灵敏检测鲈鱼（Lateolabrax japonicus）中的吡喹酮

摘要 吡喹酮（PZQ）是一种常用作杀虫剂的化合物，药物残留可能危害人体健康。在这项研究中，我们开发了一种简单、灵敏且快速的侧向流动免疫色谱法 (LFIA)，用于检测鱼 (Lateolabrax Japonicus) 中的 PZQ。我们使用酶联免疫吸附试验生产了一种灵敏的 mAb，半数抑制浓度 (2A11, IC50) 为 2.06 ng/mL。包被抗原（PZQ-HS-GA-OVA）和山羊抗小鼠 IgG 抗体分别喷洒在硝酸纤维素膜上作为测试线和对照线。在最佳条件下，在 0.01 M 磷酸盐缓冲盐水和鱼样品中，测试条上 PZQ 检测的临界值确定为 5 ng/mL，并在 5 分钟内获得结果。