ABSTRACT This study reports a fluorescence ratio immunosensor method for detecting dibutyl phthalate (DBP) sensitively and specifically using dual-emission carbon quantum dots labelled with amino-modified nucleic acid aptamers. Under optimal experimental conditions, the linearity range of the method for detecting DBP was from 12.5 to 1500 μg/L, with a limit of detection of 5.0 μg/L. Compared with detection results for two structural analogues, this method showed good specificity for DBP. Liquor samples spiked with DBP measured by this method exhibited a standard recovery of 93.2% to 112.4% with a relative standard deviation of 1.59% to 4.54%. The method was used to determine the DBP content in edible oil samples, providing results not significantly different from those obtained by gas chromatography. Compared with the single wavelength emission method, this method had a stronger anti-interference ability and provided more accurate results, so can be used for highly sensitive detection of DBP in food samples.

中文翻译：

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基于双发射碳量子点标记适体的荧光比免疫传感器检测食品样品中的邻苯二甲酸二丁酯

摘要 本研究报告了一种荧光比率免疫传感器方法，该方法使用标记有氨基修饰核酸适体的双发射碳量子点灵敏且特异性地检测邻苯二甲酸二丁酯 (DBP)。在最佳实验条件下，该方法检测DBP的线性范围为12.5～1500 μg/L，检出限为5.0 μg/L。与两种结构类似物的检测结果相比，该方法对 DBP 表现出良好的特异性。通过这种方法测得的加标 DBP 的酒样表现出 93.2% 至 112.4% 的标准回收率，相对标准偏差为 1.59% 至 4.54%。该方法用于测定食用油样品中的 DBP 含量，其结果与气相色谱法获得的结果没有显着差异。