ABSTRACT Citreoviridin (CTV) is a toxic secondary metabolite that can be found in mouldy cereals. In this study, a sensitive and ethanol-tolerant anti-CTV monoclonal antibody (6B2) was produced with a 50% inhibition concentration of 4.1 ng/mL and then labelled using gold nanoparticle (GNP). Based on competitive inhibition of the binding of CTV-COOH-BSA conjugate sprayed onto a membrane to limited GNP-labelled antibody by free CTV in the samples, an easy-to-operate immunochromatographic strip (ICS) was developed for the fast screening of CTV in rice. The results are seen as a colour change within 5-10 min. We used relatively safe ethanol instead of highly toxic methanol or chloroform as the extraction solution and found a visible detection limit of 100 μg/kg and cut-off value of 1000 μg/kg. The developed ICS assay worked well and represented a reliable, safe, fast and a highthroughput method for the detection of CTV residues in rice samples.

中文翻译：

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使用基于单克隆抗体的免疫层析试纸条法快速测定水稻中的青霉素残留

摘要 Citreoviridin (CTV) 是一种有毒的次级代谢产物，可以在发霉的谷物中找到。在这项研究中，生产了一种敏感且耐乙醇的抗 CTV 单克隆抗体 (6B2)，其 50% 抑制浓度为 4.1 ng/mL，然后使用金纳米颗粒 (GNP) 进行标记。基于样品中游离 CTV 对喷在膜上的 CTV-COOH-BSA 偶联物与有限 GNP 标记抗体的结合的竞争性抑制，开发了一种易于操作的免疫层析条 (ICS) 用于快速筛选 CTV在米饭中。结果显示为 5-10 分钟内的颜色变化。我们使用相对安全的乙醇代替剧毒的甲醇或氯仿作为提取液，发现可见检出限为 100 μg/kg，截断值为 1000 μg/kg。