ABSTRACT We aimed to determine whether dietary supplementation with spray-dried porcine plasma (SDP) is effective in mice exposed to a dual S. aureus enterotoxin B (SEB) and lipopolysaccharide (LPS) challenges inducing simultaneous inflammation in the gut and lung. Male C57BL/6 mice were fed control or SDP-supplemented diets from weaning for 14 days. At the end of the experimental period, animals received LPS intranasal followed by SEB intraperitoneal doses. The dual challenge increased leukocyte recruitment into mesenteric lymph nodes and lung tissue, the percentage of activated monocytes and neutrophils, as well as the expression of Tnf-α, Ifn-γ and Il-1β in lung tissue and jejunal mucosa, while SDP attenuated these effects. Moreover, SDP augmented the expression of Il-10, Tgf-β and Foxp3 in these tissues. Supplementation with animal plasma proteins attenuated lung and intestinal inflammation provoked by simultaneous administration of LPS and SEB toxins and increased the expression of anti-inflammatory cytokines.

中文翻译：

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动物血浆蛋白补充剂对同时发生肠道和肺部炎症的小鼠的抗炎作用

摘要 我们的目的是确定膳食补充剂喷雾干燥猪血浆 (SDP) 是否对暴露于金黄色葡萄球菌肠毒素 B (SEB) 和脂多糖 (LPS) 双重挑战的小鼠有效，从而导致肠道和肺部同时发生炎症。雄性 C57BL/6 小鼠从断奶期开始喂食对照或补充 SDP 的饮食 14 天。在实验期结束时，动物接受 LPS 鼻内给药，然后是 SEB 腹膜内给药。双重挑战增加了肠系膜淋巴结和肺组织中白细胞的募集、活化的单核细胞和中性粒细胞的百分比，以及肺组织和空肠粘膜中 Tnf-α、Ifn-γ 和 Il-1β 的表达，而 SDP 减弱了这些效果。此外，SDP 增加了这些组织中 Il-10、Tgf-β 和 Foxp3 的表达。