ABSTRACT This study was to evaluate the protective effect of fucoidan on the intestinal mucosal barrier in non-obese diabetic mice (NOD). Twenty NOD mice were randomly assigned to two groups: the control group and the fucoidan intervention group which were fed with fucoidan at a concentration of 600 mg/kg BW (body weight) per day for five weeks. Mice treated with fucoidan had a significant improvement in glycemic control, and the proportions of Th1/Th2/Th17 cells and the levels of endotoxin, DAO and D-LA were reduced. Fucoidan treatment improved intestinal barrier function by increasing ZO-1, claudin-1 and Occludin protein expression. Moreover, the expression levels of phosphorylated ERK1/2, NF-кB p65 and p38 MAPK were decreased. Our result suggested that pre-treatment with fucoidan could attenuate the inflammatory response and maintain intestinal barrier function in NOD mice. Fucoidan-based functional food may be potential for the prevention of T1DM (type 1 diabetes mellitus).

中文翻译：

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褐藻糖胶对NOD小鼠肠黏膜屏障功能的保护作用及机制

摘要 本研究旨在评估褐藻糖胶对非肥胖糖尿病小鼠 (NOD) 肠黏膜屏障的保护作用。20 只 NOD 小鼠被随机分配到两组：对照组和岩藻依聚糖干预组，它们以每天 600 mg/kg BW（体重）的浓度喂食岩藻依聚糖，持续五周。经褐藻糖胶处理的小鼠血糖控制显着改善，Th1/Th2/Th17细胞比例和内毒素、DAO和D-LA水平降低。褐藻糖胶治疗通过增加 ZO-1、claudin-1 和 Occludin 蛋白的表达来改善肠道屏障功能。此外，磷酸化的 ERK1/2、NF-кB p65 和 p38 MAPK 的表达水平降低。我们的结果表明，岩藻依聚糖预处理可以减弱 NOD 小鼠的炎症反应并维持肠道屏障功能。基于褐藻糖胶的功能性食品可能具有预防 T1DM（1 型糖尿病）的潜力。