Abstract Azolla meal was included in fish feed at different levels (10%, 20%, and 30%) and was fed to genetically-improved farmed tilapia (GIFT) for 90 days. The obtained results demonstrated that the final body weight, weight gain, and specific growth rate decreased significantly in fish fed 30% Azolla (P<0.05), while tilapia fed 10% and 20% did not differ significantly from those of the control (P>0.05). However, the feed conversion ratio increased significantly in fish fed 30% Azolla (P<0.05), while tilapia fed 10% and 20% did not differ significantly from those of the control (P>0.05). The body proximate analysis, amylase, lipase, protease, blood phagocytic index, and phagocytic and lysozyme activity were not affected by the inclusion of Azolla in tilapia diets and remained similar to those of the control group (P>0.05). The villus length of the foregut was not significantly affected by Azolla inclusion in tilapia diets (P>0.05). In the midgut, the villus length significantly (P<0.05) increased in fish fed Azolla at 20% and 30% compared to the control, with no differences from those fed at 10% (P>0.05). The villus length significantly (P<0.05) increased in the hind gut in fish fed Azolla at 30 % compared to the control, with no differences from those fed at 10% and 20% (P>0.05). The mucosal length of the tilapia foregut significantly (P<0.05) increased in fish fed Azolla at 10% compared to the control, with no differences from those fed at 20% and 30% (P>0.05). In the foregut and hindgut, the number of goblet cells significantly increased in fish fed Azolla at 3% compared to the control, with no differences from those fed at 10% and 20% (P>0.05), while in the midgut, the number of goblet cells significantly (P<0.05) increased in fish fed Azolla at 20% and 30% compared to the control, with no differences from those fed at 10% (P>0.05). Feeding tilapia with Azolla resulted in normal hematological and biochemical functions, with insignificant differences for the measured parameters except for the red blood cell count, which significantly (P<0.05) increased in fish fed Azolla at 20% compared to the control, with no differences from those fed at 20% and 30%.

中文翻译：

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添加红藻粉的鱼饲料对转基因养殖罗非鱼（Oreochromis niloticus）生长性能、消化酶活性和健康状况的影响

摘要 在鱼饲料中添加了不同水平（10%、20% 和 30%）的红萍粉，并饲喂转基因罗非鱼 (GIFT) 90 天。所得结果表明，饲喂30%满江红的鱼的最终体重、增重和比生长率显着降低（P<0.05），而饲喂10%和20%的罗非鱼与对照无显着差异（P >0.05）。然而，饲喂 30% 满江红的鱼饲料转化率显着增加（P<0.05），而饲喂 10% 和 20% 的罗非鱼与对照无显着差异（P>0.05）。身体近似分析、淀粉酶、脂肪酶、蛋白酶、血液吞噬指数以及吞噬和溶菌酶活性不受罗非鱼日粮中加入红萍的影响，与对照组相似（P>0.05）。罗非鱼日粮中添加红萍对前肠绒毛长度没有显着影响（P>0.05）。在中肠中，与对照相比，饲喂 20% 和 30% 的 Azolla 的鱼的绒毛长度显着增加（P<0.05），与饲喂 10% 的鱼没有差异（P>0.05）。与对照相比，饲喂 30% 红萍的鱼后肠中的绒毛长度显着增加（P<0.05），与饲喂 10% 和 20% 的鱼没有差异（P>0.05）。与对照相比，饲喂 10% 红萍的鱼的罗非鱼前肠粘膜长度显着增加（P<0.05），与饲喂 20% 和 30% 的鱼没有差异（P>0.05）。在前肠和后肠中，与对照组相比，饲喂 3% 的 Azolla 的鱼的杯状细胞数量显着增加，与饲喂 10% 和 20% 的鱼没有差异（P> 0.05），而在中肠中，与对照组相比，饲喂 20% 和 30% 的红萍的鱼的杯状细胞数量显着增加（P<0.05），与饲喂 10% 的鱼没有差异（P>0.05）。给罗非鱼喂食Azolla导致血液学和生化功能正常，除红细胞计数外，其他测量参数无显着差异，与对照组相比，喂食Azolla 20%的鱼显着增加（P<0.05），无差异来自那些以 20% 和 30% 喂食的人。