Bacillus sporothermodurans currently possesses one of the most highly heat-resistant spores (HRS), which can withstand ultra-high temperature (UHT) processing. Determination of multiple whole genome sequences of B. sporothermodurans provided an opportunity to perform the first comparative genome analysis between strains and with B. oleronius, B. cereus, and B. subtilis groups. In this study, five whole genome sequences of B. sporothermodurans strains, including those belonging to the HRS clone (SAD and BR12) normally isolated from UHT milk, were compared with the aforementioned Bacillus species for gene clusters responsible for heat resistance. In the phylogenomic analysis, B. sporothermodurans, with its closest phylogenetic neighbor, B. oleronius, clustered with B. thermoamylovorans and B. thermotolerans. Heat shock proteins GrpE, GroES, GroEL, and DnaK presented identical sequences for all B. sporothermodurans strains, indicating that differences in functional efficiency are not involved in the thermal resistance variations. However, comparing all species evaluated, B. sporothermodurans exhibited a different gene configuration in the chromosomal region of the heat shock protein GrpE. Furthermore, only B. sporothermodurans strains presented the stage II sporulation protein P gene located in this region. Multisequence alignment and phylogenetic analysis of the ClpB protein showed differences for HRS and non-HRS strains. The study identified ClpC, ClpE, and ClpX as the three ATPases putatively involved in protein disaggregation in B. sporothermodurans. Bacillussporothermodurans exhibits high homology with other Bacillus species in the DnaK, DnaJ, GroEL, and GroES cluster of genes involved in heat resistance. The data presented here pave the way to select and evaluate the phenotypic effects of genes putatively involved in heat resistance.

中文翻译：

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芽孢杆菌近亲芽孢杆菌及其近亲系统芽孢杆菌，油状芽孢杆菌，蜡状芽孢杆菌和枯草芽孢杆菌群的比较基因组分析。

芽孢杆菌现代芽孢杆菌目前拥有最高耐热性的孢子（HRS）之一，可以承受超高温（UHT）处理。B的多个全基因组序列的测定。sporothermodurans提供了一个机会在菌株之间和与B进行第一个比较基因组分析。oleronius，B。蜡状，和乙。枯草群。在这项研究中，将五个孢子梭菌菌株的五个全基因组序列，包括通常从超高温灭菌牛奶中分离的属于HRS克隆（SAD和BR12）的全基因组序列进行了比较。芽孢杆菌属物种负责耐热的基因簇。在种系学分析中，孢子梭菌B.及其最近的系统发生邻居B .。oleronius，与B聚集。thermoamylovorans和乙。耐热性。热激蛋白GrpE，GroES，GroEL和DnaK对所有B呈现相同的序列。孢子体存在热变形，表明功能效率的差异不涉及热阻变化。然而，比较评估所有的物种，乙。孢粉在热激蛋白GrpE的染色体区域中显示出不同的基因构型。此外，只有B。sporothermodurans菌株呈现位于该区域的II期孢子形成蛋白P基因。ClpB蛋白的多序列比对和系统发育分析表明，HRS和非HRS菌株存在差异。这项研究确定了ClpC，ClpE和ClpX是推定参与B蛋白分解的三种ATPase 。sporothermodurans。芽孢杆菌sporothermodurans表现出与其他高同源性芽孢杆菌属DnaK，DnaJ，GroEL和GroES簇中与耐热相关的基因中的物种。此处提供的数据为选择和评估可能与耐热性相关的基因的表型效应铺平了道路。