To explore the risk of antimicrobial resistant (AMR) non-typhoidal Salmonella during asymptomatic infection passage between pet dogs and human caregivers in Khon Kaen, Thailand, one hundred forty paired fecal samples (n = 280) were obtained from companion dogs and their human caregivers, interviewed from 140 households during 2019–2020. The purified Salmonella isolates were serotype-identified and tested for antimicrobial resistance against ampicillin, ciprofloxacin, chloramphenicol, nalidixic acid, streptomycin, sulfamethoxazole–trimethoprim, and tetracycline. The homologous Salmonella isolate pairs (suggesting Salmonella infections may have been due to passage between each one of the pair, or derived from the same source) were subsequently characterized by serotype screening, pulsed field gel electrophoresis (PFGE), and Synchrotron Fourier transform infrared spectroscopy (SR-FTIR). The Salmonella prevalence observed in dogs, 12.86% (18/140), was not significantly different from that observed in humans, 17.86% (25/140) using McNemar’s test. The AMR patterns (the patterns among the isolates of pet dogs and caregivers) and the serotypes (thirteen serotypes with 18 isolates from pet dogs plus thirteen serotypes with 25 isolates from humans) between pet dogs and humans were not significantly different using Pearson’s chi-squared test. The homologous Salmonella isolates from the Salmonella-present households was 5.13% (2/39). This study demonstrated that the hypothesis that there is a high risk of Salmonella infection passage between dogs and humans with close contact in Khon Kaen is doubtful. Only 5.13% of homologous Salmonella isolates from Salmonella-present households were found in Khon Kaen, Thailand, although the prevalence of Salmonella-positive samples, serotypes, and antimicrobial resistance patterns were quite similar among the two populations.

中文翻译：

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在泰国孔敬的宠物狗及其人类护理人员之间的无症状感染过程中，抗药性非伤寒沙门氏菌的风险。

为了探索泰国孔敬的宠物犬与人类看护者之间无症状感染传播过程中抗菌素耐药（AMR）非伤寒沙门氏菌的风险，从伴侣犬及其人类看护者中获得了一百四十对粪便样本（n = 280）。在2019–2020年期间接受了140户家庭的采访。对纯化的沙门氏菌分离株进行血清型鉴定，并测试其对氨苄青霉素，环丙沙星，氯霉素，萘啶酸，链霉素，磺胺甲恶唑–甲氧苄啶和四环素的抗药性。同源沙门氏菌分离株对（建议沙门氏菌随后可能通过血清型筛查，脉冲场凝胶电泳（PFGE）和Synchrotron傅里叶变换红外光谱（SR-FTIR）来表征感染，这可能是由于这对病毒之间的通过或源于同一源。使用McNemar检验在狗中观察到的沙门氏菌患病率为12.86％（18/140），与在人类中观察到的沙门氏菌患病率没有明显差异，为17.86％（25/140）。使用皮尔逊卡方检验，宠物狗和人之间的AMR模式（爱犬和看护者分离株之间的模式）和血清型（爱犬的18种分离株，其中18株来自人类，以及13种血清型和25分离株来自人）。测试。同源沙门氏菌从隔离沙门氏菌当前家庭为5.13％（2/39）。这项研究表明，在孔敬地区密切接触的人与狗之间存在沙门氏菌感染的高风险这一假设是可疑的。尽管沙门氏菌阳性样本，血清型和抗菌素耐药性在两个人群中普遍存在，但在泰国孔敬的沙门氏菌目前家庭中，只有5.13％的沙门氏菌分离株。