Background: To characterize the cellular composition (platelets, erythrocytes, and leukocytes) and determine platelet-derived growth factor isoform BB (PDGF-BB) concentration in canine leukocyte- and platelet rich plasma (L-PRP) produced using a commercial semi-automated closed system. Methods: Twenty milliliters of citrated whole blood were obtained from 30 healthy un-sedated canine blood donors and processed using a semi-automated completely closed commercial system (CPUNT 20, Eltek group, Casale Monferrato, Alessandria, Italy) according to the manufacturer’s instructions. Erythrocyte, leukocyte, and platelet counts were determined in both whole blood (WB) and resultant L-PRP. The PDGF-BB concentration was evaluated after bovine thrombin activation of 10 L-PRP samples. Results: This commercial system produced on average 2.3 ± 0.7 mL of L-PRP containing a high concentration of platelets (767,633 ± 291,001 μL, p < 0.001), with a 4.4 fold increase in platelet count, lower concentration of erythrocytes (528,600 ± 222,773 μL, p < 0.001) and similar concentration of leukocytes (8422 ± 6346 μL, p = 0.9918) compared with WB. L-PRP had an average of 3442 ± 2061 pg/mL of PDGF-BB after thrombin activation. Neutrophils, lymphocytes and monocytes average percent content in L-PRP was 14.8 ± 13.2, 71.7 ± 18.5 and 10.7 ± 6.4, respectively. Conclusion: Sterile canine L-PRP prepared using this semi-automated closed system is easy to obtain, produces a significant increase in platelet count compared to WB and contains a detectable concentration of PDGF-BB after activation. Additional in vitro and in vivo studies are needed to assess inflammatory markers concentration and the therapeutic efficacy of this L-PRP in dogs.

中文翻译：

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半自动化的商用封闭系统在狗体内产生自体白细胞和富含血小板的血浆（l-prp）的功效：初步研究。

背景：表征细胞成分（血小板，红细胞和白细胞），并确定使用商业半自动化生产的犬白细胞和富含血小板的血浆（L-PRP）中血小板衍生的生长因子同工型BB（PDGF-BB）的浓度封闭系统。方法：从30个健康的非镇静犬献血者中获取20毫升柠檬酸全血，并根据制造商的说明使用半自动完全封闭的商用系统（CPUNT 20，Eltek group，Casale Monferrato，Alessandria，意大利）进行处理。确定全血（WB）和所得L-PRP的红细胞，白细胞和血小板计数。牛凝血酶活化10个L-PRP样品后评估PDGF-BB浓度。结果：此商业系统平均产生2.3±0。p <0.001），血小板计数增加4.4倍，红细胞浓度较低（528,600±222,773μL，p <0.001）和白细胞浓度相似（8422±6346μL，p= 0.9918）与WB相比。凝血酶激活后，L-PRP的PDGF-BB平均为3442±2061 pg / mL。L-PRP中的中性粒细胞，淋巴细胞和单核细胞的平均百分比含量分别为14.8±13.2、71.7±18.5和10.7±6.4。结论：使用半自动封闭系统制备的无菌犬L-PRP易于获得，与WB相比，血小板计数显着增加，并且在激活后可检测到PDGF-BB的浓度。需要其他的体外和体内研究来评估炎症标志物的浓度和该L-PRP在犬中的治疗功效。