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A natural oil increases specific anti-OVA IgG levels and induces a cellular immune response combined with aluminum hydroxide.
Journal of Leukocyte Biology ( IF 3.6 ) Pub Date : 2020-08-03 , DOI: 10.1002/jlb.3ab0720-093rr Noelia Anahí Campra 1, 2 , Ivana Dalila Montironi 1, 2 , Elina Beatriz Reinoso 1, 2 , José Raviolo 3 , Federico Ruiz Moreno 4, 5 , Belkys Maletto 4, 5 , Laura Noelia Cariddi 1, 2
Journal of Leukocyte Biology ( IF 3.6 ) Pub Date : 2020-08-03 , DOI: 10.1002/jlb.3ab0720-093rr Noelia Anahí Campra 1, 2 , Ivana Dalila Montironi 1, 2 , Elina Beatriz Reinoso 1, 2 , José Raviolo 3 , Federico Ruiz Moreno 4, 5 , Belkys Maletto 4, 5 , Laura Noelia Cariddi 1, 2
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The efficacy of certain vaccines is improved by the use of adjuvants. Nowadays, the development of new, effective, and safe adjuvants that stimulate the innate immune response is researched. In this context, medicinal plants appear as a suitable alternative. Minthostachys verticillata essential oil (EO) has demonstrated the ability to modulate mechanisms of the innate immune response. Thus, the present work aimed to evaluate the EO adjuvant effect on humoral and cellular immunity, coadministered with OVA as antigen. The chemical analysis of EO by gas chromatography–mass spectrometry revealed a predominant pulegone–menthone chemotype. EO (1.25, 2.5, or 5.0 mg/ml) did not alter the viability of murine fibroblasts (3T3 cell line) neither showed signs of toxicity in Balb/c mice inoculated subcutaneously. The serum of mice immunized with OVA + EO showed increased levels of anti‐OVA‐specific antibodies of IgG1 subclass compared with the mice immunized with OVA alone revealing an adjuvant effect of EO. The delayed type hypersensitivity showed that the combination OVA + Al(OH)3 + EO was the best to induce a cellular immune response that extended until 48 h postinjection of OVA. M. verticillata EO appears as a new, safe, and effective adjuvant, which should continue to be studied for their possible future incorporation into vaccine formulations.
中文翻译：
天然油会增加特定的抗OVA IgG水平，并与氢氧化铝结合诱导细胞免疫反应。
通过使用佐剂可以提高某些疫苗的功效。如今，正在研究刺激先天免疫应答的新型，有效和安全的佐剂。在这种情况下，药用植物似乎是合适的选择。拟南芥精油（EO）已证明具有调节先天免疫应答机制的能力。因此，本工作旨在评估EO佐剂与OVA作为抗原共同对体液和细胞免疫的辅助作用。通过气相色谱-质谱法对EO进行化学分析，结果显示主要的普勒热酮-薄荷酮化学型。EO（1.25、2.5或5.0 mg / ml）不会改变鼠成纤维细胞（3T3细胞系）的活力，在皮下接种的Balb / c小鼠中也未显示毒性迹象。与仅用OVA免疫的小鼠相比，用OVA + EO免疫的小鼠的血清显示出更高的IgG1亚型抗OVA特异性抗体水平，从而显示了EO的佐剂作用。迟发型超敏反应表明，OVA + Al（OH）3组合 + EO是诱导细胞免疫反应的最佳方法，该反应可延长至OVA注射后48小时。verticillata EO似乎是一种新的，安全的和有效的佐剂，应继续研究其未来是否可能掺入疫苗制剂中。
更新日期：2020-08-03
中文翻译：
天然油会增加特定的抗OVA IgG水平，并与氢氧化铝结合诱导细胞免疫反应。
通过使用佐剂可以提高某些疫苗的功效。如今，正在研究刺激先天免疫应答的新型，有效和安全的佐剂。在这种情况下，药用植物似乎是合适的选择。拟南芥精油（EO）已证明具有调节先天免疫应答机制的能力。因此，本工作旨在评估EO佐剂与OVA作为抗原共同对体液和细胞免疫的辅助作用。通过气相色谱-质谱法对EO进行化学分析，结果显示主要的普勒热酮-薄荷酮化学型。EO（1.25、2.5或5.0 mg / ml）不会改变鼠成纤维细胞（3T3细胞系）的活力，在皮下接种的Balb / c小鼠中也未显示毒性迹象。与仅用OVA免疫的小鼠相比，用OVA + EO免疫的小鼠的血清显示出更高的IgG1亚型抗OVA特异性抗体水平，从而显示了EO的佐剂作用。迟发型超敏反应表明，OVA + Al（OH）3组合 + EO是诱导细胞免疫反应的最佳方法，该反应可延长至OVA注射后48小时。verticillata EO似乎是一种新的，安全的和有效的佐剂，应继续研究其未来是否可能掺入疫苗制剂中。
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