Sensitivity of a PER.C6® cell line to bis(2,4-di-tert-butylphenyl)phosphate and evaluation of a new, biocompatible single-use film.,Biotechnology Progress

当前位置： X-MOL 学术 › Biotechnol. Prog. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Sensitivity of a PER.C6® cell line to bis(2,4-di-tert-butylphenyl)phosphate and evaluation of a new, biocompatible single-use film.
Biotechnology Progress ( IF 2.5 ) Pub Date : 2020-08-03 , DOI: 10.1002/btpr.3060
Joshua R Jachuck _{1,

2} , Soumya Mohanan Krishnathu ₁ , Jeffrey E Landau ₁ , Hsu-Feng Ko ₁ , Ravi Bhatia ₁

Affiliation

Single‐use technologies have brought numerous advantages to the biopharmaceutical industry. In particular, single‐use bags made from multi‐layered polymeric films have been adopted for cell culture and liquid handling operations in place of traditional stainless‐steel systems. Despite the advantages, leachable compounds originating from the film's materials of construction present a new challenge. In 2013, bis(2,4‐di‐tert‐butylphenyl)phosphate (bDtBPP) was identified as a common leachable from several single‐use bags that is detrimental to the growth and viability of many Chinese hamster ovary (CHO) cell lines. While much work has been completed to characterize CHO cell sensitivity to bDtBPP, little has been done to characterize its impact on other important production cell lines, particularly PER.C6®. This publication investigates inconsistent cell growth observed in a PER.C6® cell line during bioprocess development. The growth inhibition was linked to leachable migration from Bioclear™ 10, a single‐use film from Cytiva (formerly GE Healthcare) that was used for cell expansion. It was shown that the PER.C6® cells displayed a sensitivity to bDtBPP, comparable to that observed in sensitive CHO cell lines. Finally, biocompatibility of PER.C6® with Cytiva's new Fortem film was evaluated, demonstrating that Fortem™ film is a suitable single‐use technology for culturing PER.C6® cells.

更新日期：2020-08-03

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11