Production of β‐glucosidase was investigated in nine isolates of Armillaria representing four species found in Europe: Armillaria mellea and Armillaria ostoyae, considered to be pathogenic and moderately pathogenic, respectively, and Armillaria. gallica and Armillaria cepistipes, both considered to be non‐pathogenic. β‐glucosidase was predominantly produced in the rhizomorphs, while the vegetative mycelium produced only a low amount of enzyme. Pachlewski's medium containing ammonium tartrate, glucose, maltose and thiamine was very efficient in promoting differentiation and growth of rhizomorphs. In A. ostoyae and A. cepistipes, a large proportion of β‐glucosidase production was endocellular, but the rhizomorphs of all species excreted significant amounts of the enzyme in the culture medium once they had grown. The pathogenic species A. ostoyae and A. mellea excreted significantly more β‐glucosidase than the non‐pathogenic species, indicating that this enzyme might play a key role in pathogenicity. Native intracellular β‐glucosidases were visualized as three bands at 470, 164 and 82 kDa on polyacrylamide gradient gels. The native excreted enzymes exhibited two, three or four bands depending on the isolates, with molecular weights ranging from 170 to 400 kDa.

中文翻译：

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欧洲蜜环菌属物种生产β-葡萄糖苷酶

在代表欧洲发现的四个物种的9株蜜环菌中研究了β-葡萄糖苷酶的产生：蜜环菌（melillaria mellea）和蜜环菌（Armillaria ostoyae），分别被认为具有致病性和中度致病性，以及蜜环菌。高卢和蜜cepistipes，都被认为是不致病的。β-葡萄糖苷酶主要产生于根状茎中，而营养菌丝体仅产生少量酶。含有酒石酸铵，葡萄糖，麦芽糖和硫胺素的Pachlewski培养基非常有效地促进了根形的分化和生长。在A. ostoyae和A. cepistipes，很大一部分β-葡萄糖苷酶的产生是在细胞内，但所有物种的根形一旦长大，就会在培养基中分泌出大量的酶。病原菌ostostae和A. mellea比非病原菌分泌更多的β-葡萄糖苷酶，表明该酶可能在致病性中起关键作用。在聚丙烯酰胺梯度凝胶上，天然细胞内β-葡萄糖苷酶在470、164和82 kDa处显示为三个条带。根据分离物，天然分泌的酶表现出两个，三个或四个条带，分子量范围为170至400 kDa。